ArticleJournal of molecular biology2020
The Structural Basis of T4 Phage Lysis Control: DNA as the Signal for Lysis Inhibition.
Article in Journal of molecular biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed.
- Phage therapy: from basic biology to clinical application.Nature reviews. Microbiology · 2026Review
- Host physiology-dependent lysis timing shapes bacteriophage competition under nutrient fluctuations.mSystems · 2026Article
- Prophage: agent provocateur?Archives of microbiology · 2026Review
- The Lysis cassette of jumbophage PhiKZ.Scientific reports · 2026Article
- Optical Density-Based Methods in Phage Biology: Titering, Lysis Timing, Host Range, and Phage-Resistance Evolution.Viruses · 2025Review
- Phage N4 uses a SAR endolysin-holin system for host cell lysis.bioRxiv : the preprint server for biology · 2025Article
- Antibiotic-induced morphological changes enhance phage predation.PLoS pathogens · 2025Article
- Genetic insights into novel lysis suppression by phage CSP1 in Escherichia coli.Journal of microbiology (Seoul, Korea) · 2025Article
- The short cytoplasmic region of phage T4 holin is essential for the transition from impermeable membrane protein complexes to permeable pores.Frontiers in microbiology · 2025Article
- Bacteriophage titering by optical density means: KOTE assays.Open life sciences · 2025Review
- Characterization of holins, the membrane proteins of coliphage ASEC2201: a genomewideFrontiers in microbiology · 2025Article
- Article
- A dimeric holin/antiholin complex controls lysis by phage T4.Frontiers in microbiology · 2024Article
- More than just pattern recognition: Prediction of uncommon protein structure features by AI methods.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- The Burden of Survivors: How Can Phage Infection Impact Non-Infected Bacteria?International journal of molecular sciences · 2023Review
- Interaction between Phage T4 Protein RIII and Host Ribosomal Protein S1 Inhibits Endoribonuclease RegB Activation.International journal of molecular sciences · 2022Article
- Evaluating the Phenotypic and Genomic Characterization of Some Egyptian Phages Infecting Shiga Toxin-ProducingBiology · 2022Article
- Comparative genomics of Acinetobacter baumannii and therapeutic bacteriophages from a patient undergoing phage therapy.Nature communications · 2022Article
- Characterization ofFrontiers in microbiology · 2022Article
- Bacteriophage-encoded lethal membrane disruptors: Advances in understanding and potential applications.Frontiers in microbiology · 2022Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Optimal phage propagation depends on the regulation of the lysis of the infected host cell. In T4 phage infection, lysis occurs when the holin protein (T) forms lesions in the host membrane. However, the lethal function of T can be blocked by an antiholin (RI) during lysis inhibition (LIN). LIN sets if the infected cell undergoes superinfection, then the lysis is delayed until host/phage ratio becomes more favorable for the release of progeny. It has been thought that a signal derived from the superinfection is required to activate RI. Here we report structures that suggest a radically different model in which RI binds to T irrespective of superinfection, causing it to accumulate in a membrane as heterotetrameric 2RI-2T complex. Moreover, we show the complex binds non-specifically to DNA, suggesting that the gDNA from the superinfecting phage serves as the LIN signal and that stabilization of the complex by DNA binding is what defines LIN. Finally, we show that soluble domain of free RI crystallizes in a domain-swapped homotetramer, which likely works as a sink for RI molecules released from the RI-T complex to ensure efficient lysis. These results constitute the first structural basis and a new model not only for the historic LIN phenomenon but also for the temporal regulation of phage lysis in general.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.