ArticleParasitology research2020
A phylogenetic study of the cecal amphistome Zygocotyle lunata (Trematoda: Zygocotylidae), with notes on the molecular systematics of Paramphistomoidea.
Article in Parasitology research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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8 citing papers in PubMed.
- Morphological and molecular identification of amphistomes occurring in cattle in Zimbabwe.Parasitology research · 2026Article
- Catadiscus marielaosornae n. sp. (Digenea: Diplodiscidae) infecting the intestine of Peters' thin-toed frog, Leptodactylus petersii (Steindachner) (Anura: Leptodactylidae) from the Yahuarcaca Lake System, Amazon River (Leticia, Colombia), with phylogenetic analysis.Systematic parasitology · 2025Article
- Molecular analysis supports the morphological identification of the amphistome Pseudodiscus collinsi (Paramphistomoidea: Platyhelminthes) in wild elephants of Sri Lanka.Veterinary research communications · 2025Article
- Evolutionary affinities and morphological characterization of the enigmatic Zonocotyle bicaecata (Trematoda: Paramphistomoidea: Zonocotylidae) from the Upper Paraná River basin.Systematic parasitology · 2024Article
- Size does not matter: molecular phylogeny reveals one of the largest trematodes from vertebrates, the enigmaticInternational journal for parasitology. Parasites and wildlife · 2022Review
- Morphological and molecular characterization ofInternational journal for parasitology. Parasites and wildlife · 2022Article
- Hidden in plain sight: novel molecular data reveal unexpected genetic diversity among paramphistome parasites (Digenea: Paramphistomoidea) of European water frogs.Parasitology · 2022Article
- Exploring Neotropical anuran parasites: a morphological, life cycle and phylogenetic study ofParasitology · 2021Article
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7 authors.
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Abstract
Zygocotyle lunata inhabits the caecum of birds and mammals from the American continent. This amphistome parasite is easily maintained in the laboratory and serves as a model organism in life-cycle studies, but it has seldom been studied using molecular data. Neither the position of Z. lunata in the superfamily Paramphistomoidea nor the monophyly of the Zygocotylidae has been evaluated with molecular phylogenetic methods. In the present study, adult specimens of Z. lunata obtained experimentally in mice from Brazil were submitted to molecular studies. Partial sequences of nuclear (1261 bp of 28S and 418 bp of 5.8S-ITS-2) and mitochondrial (1410 bp of cytochrome c oxidase 1, cox1) markers were compared with published data. In the most well-resolved phylogeny, based on 28S sequences, Z. lunata clustered in a well-supported clade with Wardius zibethicus, the only other species currently included in the Zygocotylidae, thus confirming the validity of this family. Divergence of 28S sequences between these species was 2.2%, which falls in the range of intergeneric variation (0.9-5.6%) observed in the other two monophyletic groups in the 28S tree, i.e., representatives of Gastrodicidae and Neotropical cladorchiids (Cladorchiidae). Analysis of ITS-2 and two parts of the cox1 gene placed Z. lunata within poorly resolved clades or large polytomies composed of several paramphistomoid families, without clarifying higher-level phylogenetic relationships. The cox1 of a Brazilian isolate of Z. lunata is 99.6% similar to a Canadian isolate, confirming the pan-American distribution of the species. Finally, our phylogenetic reconstructions of Paramphistomoidea revealed a complex scenario in the taxonomic composition of some amphistome families, which highlights a need for further integrative studies that will likely result in rearrangements of traditional morphology-based classifications.
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