ArticleCommunications biology2020
Structure determination of the HgcAB complex using metagenome sequence data: insights into microbial mercury methylation.
Article in Communications biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 47 citations in OpenAlex.
- Metagenomics reveals diverse community of putative mercury methylators across different biogeochemical niches in Sansha Yongle blue hole.Marine life science & technology · 2026Article
- Unveiling Hidden Mercury and Methylmercury Sources: The Role of Submarine Groundwater Discharge in Coastal Lagoons.Environmental science & technology · 2025Article
- S-adenosyl-L-methionine is the unexpected methyl donor for the methylation of mercury by the membrane-associated HgcAB complex.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- The origin of methyl group in methanogen-mediated mercury methylation: From the Wolfe cycle.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Functional Genes and Transcripts Indicate the Existent and Active Microbial Mercury-Methylating Community in Mangrove Intertidal Sediments of an Urbanized Bay.Microorganisms · 2024Article
- Recent advance of microbial mercury methylation in the environment.Applied microbiology and biotechnology · 2024Review
- Review
- Transcriptional Control ofApplied and environmental microbiology · 2023Article
- Accurate prediction by AlphaFold2 for ligand binding in a reductive dehalogenase and implications for PFAS (per- and polyfluoroalkyl substance) biodegradation.Scientific reports · 2023Article
- Potential for mercury methylation by Asgard archaea in mangrove sediments.The ISME journal · 2023Article
- A consensus protocol for the recovery of mercury methylation genes from metagenomes.Molecular ecology resources · 2023Article
- Article
- Expression Levels ofEnvironmental science & technology · 2022Article
- Long-term mercury contamination does not affect the microbial gene potential for C and N cycling in soils but enhances detoxification gene abundance.Frontiers in microbiology · 2022Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
Abstract
Bacteria and archaea possessing the hgcAB gene pair methylate inorganic mercury (Hg) to form highly toxic methylmercury. HgcA consists of a corrinoid binding domain and a transmembrane domain, and HgcB is a dicluster ferredoxin. However, their detailed structure and function have not been thoroughly characterized. We modeled the HgcAB complex by combining metagenome sequence data mining, coevolution analysis, and Rosetta structure calculations. In addition, we overexpressed HgcA and HgcB in Escherichia coli, confirmed spectroscopically that they bind cobalamin and [4Fe-4S] clusters, respectively, and incorporated these cofactors into the structural model. Surprisingly, the two domains of HgcA do not interact with each other, but HgcB forms extensive contacts with both domains. The model suggests that conserved cysteines in HgcB are involved in shuttling Hg
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