ArticleScientific reports2020
hFRUIT: An optimized agent for optical clearing of DiI-stained adult human brain tissue.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 19 citations in OpenAlex.
- A uniform tissue-clearing framework and mesoSPIM-ultra enable cm-scale single-neuron tracing.bioRxiv : the preprint server for biology · 2026Article
- activeDISCO enables large-scale exploration of human brain anatomy by light sheet microscopy.Communications biology · 2026Article
- Mapping intralobar fiber connections in the human occipital lobe by tracer electrophoresis.Brain structure & function · 2026Article
- PRIAMOS: A technique for mixing embedding media for freely adjusting pH value and refractive index (RI) for optical clearing (OC) of whole tissue samples.Journal of microscopy · 2025Article
- ht-MASH: a high-throughput, cost-effective, and robust protocol for microscopic 3D imaging of human angio- and cytoarchitecture in large human brain samples.Anatomical science international · 2025Article
- Advances in tissue optical clearing for 3D imaging in large animal.Frontiers of optoelectronics · 2025Review
- Unlocking the potential of large-scale 3D imaging with tissue clearing techniques.Microscopy (Oxford, England) · 2025Review
- FLUID: a fluorescence-friendly lipid-compatible ultrafast clearing method.Biomedical optics express · 2024Article
- Over 30 Years of DiI Use for Human Neuroanatomical Tract Tracing: A Scoping Review.Biomolecules · 2024Article
- Optimized single-step optical clearing solution for 3D volume imaging of biological structures.Communications biology · 2022Article
- Testing the suitability of neuroanatomical tracing method in human fetuses with long years of postmortem delay.Surgical and radiologic anatomy : SRA · 2022Article
- Physical and chemical mechanisms of tissue optical clearing.iScience · 2021Review
- Optical Tissue Clearing: Illuminating Brain Function and Dysfunction.Theranostics · 2021Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here, we describe a new immersion-based clearing method suitable for optical clearing of thick adult human brain samples while preserving its lipids and lipophilic labels such as 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI). This clearing procedure is simple, easy to implement, and allowed for clearing of 5 mm thick human brain tissue samples within 12 days. Furthermore, we show for the first time the advantageous effect of the Periodate-Lysine-Paraformaldehyde (PLP) fixation as compared to the more commonly used 4% paraformaldehyde (PFA) on clearing performance.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.