ArticleNature communications2020
Holistic engineering of cell-free systems through proteome-reprogramming synthetic circuits.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 37 citations in OpenAlex.
- Article
- Developing anACS synthetic biology · 2025Article
- Designer artificial environments for membrane protein synthesis.Nature communications · 2025Article
- Cell membrane-derived nanovesicles as extracellular vesicle-mimetics in wound healing.Materials today. Bio · 2025Review
- Cell-Free Gene Expression: Methods and Applications.Chemical reviews · 2025Review
- Context-dependent redesign of robust synthetic gene circuits.Trends in biotechnology · 2024Review
- Cracking the Code: Enhancing Molecular Tools for Progress in Nanobiotechnology.ACS applied bio materials · 2024Review
- Engineering cell-free systems by chemoproteomic-assisted phenotypic screening.RSC chemical biology · 2024Article
- Synthetic control of living cells by intracellular polymerization.Trends in biotechnology · 2024Review
- Toward predictive engineering of gene circuits.Trends in biotechnology · 2023Review
- MIRELLA: a mathematical model explains the effect of microRNA-mediated synthetic genes regulation on intracellular resource allocation.Nucleic acids research · 2023Article
- The Use of Cell-free Protein Synthesis to Push the Boundaries of Synthetic Biology.Biotechnology and bioprocess engineering : BBE · 2023Review
- What remains from living cells in bacterial lysate-based cell-free systems.Computational and structural biotechnology journal · 2023Review
- Multiple Gene Expression in Cell-Free Protein Synthesis Systems for Reconstructing Bacteriophages and Metabolic Pathways.Microorganisms · 2022Review
- High-Throughput Experimentation Using Cell-Free Protein Synthesis Systems.Methods in molecular biology (Clifton, N.J.) · 2022Article
- An integrated in vivo/in vitro framework to enhance cell-free biosynthesis with metabolically rewired yeast extracts.Nature communications · 2021Article
- Analysis of the Innovation Trend in Cell-Free Synthetic Biology.Life (Basel, Switzerland) · 2021Article
- Improving cell-free glycoprotein synthesis by characterizing and enriching native membrane vesicles.Nature communications · 2021Article
- Anaerobic Conditioning ofACS synthetic biology · 2021Article
- Optimising protein synthesis in cell-free systems, a review.Engineering biology · 2021Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Synthetic biology has focused on engineering genetic modules that operate orthogonally from the host cells. A synthetic biological module, however, can be designed to reprogram the host proteome, which in turn enhances the function of the synthetic module. Here, we apply this holistic synthetic biology concept to the engineering of cell-free systems by exploiting the crosstalk between metabolic networks in cells, leading to a protein environment more favorable for protein synthesis. Specifically, we show that local modules expressing translation machinery can reprogram the bacterial proteome, changing the expression levels of more than 700 proteins. The resultant feedback generates a cell-free system that can synthesize fluorescent reporters, protein nanocages, and the gene-editing nuclease Cas9, with up to 5-fold higher expression level than classical cell-free systems. Our work demonstrates a holistic approach that integrates synthetic and systems biology concepts to achieve outcomes not possible by only local, orthogonal circuits.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.