Evidence map›Paper›PMID 32560807›Full record

ArticleMethods in enzymology2020

Chemiluminescent sensors for quantitation of the bacterial second messenger cyclic di-GMP.

Wyatt A Anderson, Andrew B Dippel, Michael M Maiden, Christopher M Waters, Ming C Hammond

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in enzymology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. bioRxiv : the preprint server for biology · 2026
    Article
  2. Optical Sensors for Bacterial Detection.Sensors (Basel, Switzerland) · 2023
    Review
  3. Analyzing signaling activity and function in hematopoietic cells.The Journal of experimental medicine · 2021
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Wyatt A AndersonDepartment of Chemistry and Henry Eyring Center for Cell and Genome Science, University of Utah, Salt Lake City, UT, United States; Department of Chemistry, University of California, Berkeley, Berkeley, CA, United States.
Andrew B DippelDepartment of Chemistry and Henry Eyring Center for Cell and Genome Science, University of Utah, Salt Lake City, UT, United States; Department of Chemistry, University of California, Berkeley, Berkeley, CA, United States.
Michael M MaidenDepartments of Microbiology and Molecular Genetics and The BEACON Center for The Study of Evolution in Action, Michigan State University, East Lansing, MI, United States.
Christopher M WatersDepartments of Microbiology and Molecular Genetics and The BEACON Center for The Study of Evolution in Action, Michigan State University, East Lansing, MI, United States.
Ming C HammondDepartment of Chemistry and Henry Eyring Center for Cell and Genome Science, University of Utah, Salt Lake City, UT, United States; Department of Chemistry, University of California, Berkeley, Berkeley, CA, United States. Electronic address: mingch@chem.utah.edu.

Funding

Enabling High-Throughput Analysis and Single-Cell Imaging of Bacterial SignalsR01GM124589 · NIGMS · UTAH STATE HIGHER EDUCATION SYSTEM--UNIVERSITY OF UTAH · PI HAMMOND, MING CHEN · 2017 to 2025
$2.8M
From structure to systems: Understanding cyclic di-GMP control of transcriptionR01GM109259 · NIGMS · MICHIGAN STATE UNIVERSITY · PI WATERS, CHRISTOPHER M · 2015 to 2019
$2.0M
NIGMS NIH HHS R01 GM109259NIGMS NIH HHS R01 GM124589
6 · The paper itself

Abstract

Chemiluminescent biosensors have been developed and broadly applied to mammalian cell systems for studying intracellular signaling networks. For bacteria, biosensors have largely relied on fluorescence-based systems for quantitating signaling molecules, but these designs can encounter issues in complex environments due to their reliance on external illumination. In order to circumvent these issues, we designed the first ratiometric chemiluminescent biosensors for studying a key bacterial second messenger, cyclic di-GMP. We have shown recently that these biosensors function both in vitro and in vivo for detecting changes in cyclic di-GMP levels. In this chapter, we present a practical and broadly applicable method for high-throughput quantitation of cyclic di-GMP in bacterial cell extracts using the high affinity biosensor tVYN-TmΔ that could serve as the "Bradford assay" equivalent for this bacterial signaling molecule.

Indexed as

Biosensing TechniquesVibrio choleraeAnimalsBacteriaBacterial ProteinsBiofilmsCyclic GMPGene Expression Regulation, BacterialSecond Messenger SystemsSignal TransductionBacterial ProteinsCyclic GMPBioluminescenceCell extractsRatiometric biosensorVibrio cholerae

Identifiers

PMID32560807
PMCPMC8238376

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.