Evidence map›Paper›PMID 32560797›Full record

ArticleMethods in enzymology2020

Bioluminescence imaging in mice with synthetic luciferin analogues.

Xincai Ji, Spencer T Adams, Stephen C Miller

Open access · greenAbstract read
In one paragraph

Article in Methods in enzymology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
3.9field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 18 citations in OpenAlex.

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  15. How to Select Firefly Luciferin Analogues for In Vivo Imaging.International journal of molecular sciences · 2021
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Xincai JiDepartment of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA, United States.
Spencer T AdamsDepartment of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA, United States.
Stephen C MillerDepartment of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA, United States. Electronic address: stephen.miller@umassmed.edu.
University of Massachusetts Chan Medical School · US

Funding

Enhanced synthetic luciferin substrates for firefly luciferaseR01EB013270 · NIBIB · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI MILLER, STEPHEN C. · 2011 to 2020
$2.9M
Illuminating serine hydrolase activityR01DA039961 · NIDA · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI MILLER, STEPHEN C. · 2015 to 2018
$1.9M
NIBIB NIH HHS R01 EB013270NIDA NIH HHS R01 DA039961
6 · The paper itself

Abstract

Luciferase enzymes from bioluminescent organisms can be expressed in mice, enabling these rodents to glow when treated with a corresponding luciferin substrate. Light emission occurs where the expression of the genetically-encoded luciferase overlaps with the biodistribution of the administered small molecule luciferin. Here we discuss differences between firefly luciferin analogues for bioluminescence imaging, focusing on transgenic and adeno-associated virus (AAV)-transduced mice.

Indexed as

Firefly LuciferinLuminescent MeasurementsAnimalsLuciferasesLuciferases, FireflyMiceTissue DistributionFirefly LuciferinLuciferasesLuciferases, FireflyAAVCoelenterazineCycLuc1FAAHFireflyGFAPLuciferaseNanoLucPHP.eBTransgenic

Identifiers

PMID32560797
PMCPMC8345814
OpenAlexW3030290269

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.