ArticleBMC plant biology2020
CRISPR/Cas9-targeted mutagenesis of the tomato susceptibility gene PMR4 for resistance against powdery mildew.
Article in BMC plant biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
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76 citing papers in PubMed.
- Molecular Targets and Trait Innovation in CRISPR-Edited Vegetable Crops: An Up-to-Date Review (2020-2026).Plants (Basel, Switzerland) · 2026Review
- Genome Editing in Solanaceae: Harnessing CRISPR-Cas Technology for Precision Crop Improvement.Plants (Basel, Switzerland) · 2026Review
- Advances in mutant characterization for detecting causal mutations in crop plants.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Review
- Advances in CRISPR Plant Applications.International journal of molecular sciences · 2026Review
- Toward sustainable control of phyto-nematodes: integrating lessons from crops to advance genetic modification in tomato.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Review
- Elucidating putative novel functions of the AINTEGUMENTA-LIKE 7 transcription factor in clubroot resistance in Arabidopsis.Plant molecular biology · 2026Article
- From Lab to Field: CRISPRing Major CultivatedInternational journal of molecular sciences · 2026Review
- Establishment of Efficient CRISPR-Cas9 PEG-Mediated DNA-Free Genome Editing Through Ribonucleoproteins Method in Hexaploid Sweetpotato (Plants (Basel, Switzerland) · 2026Article
- Next-Generation Precision Breeding in Peanut (Insects · 2026Review
- Next-generation CRISPR systems and advanced delivery paradigms for precision crop improvement: a comparative case study in sugar beet (Frontiers in plant science · 2026Review
- Solanum tuberosum glucan synthase-like 05 is involved in root-knot nematode Meloidogyne hapla parasitism.Scientific reports · 2025Article
- The Enhancement of Fungal Disease Resistance in Major Staple Crops Using CRISPR-Cas Technology.Genes · 2025Review
- Turning Susceptibility into Strength: A New Era of Durable Resistance in Plants Through Genome Editing.Plants (Basel, Switzerland) · 2025Review
- Allelic value in gene regulation-implications for gene editing.Annals of botany · 2025Article
- Mutation of PUB21 in tomato leads to reduced susceptibility to necrotrophic fungi.BMC plant biology · 2025Article
- Review
- Recent advances in innovative strategies for plant disease resistance breeding.Frontiers in plant science · 2025Review
- Unlocking male sterility in horticultural crops through gene editing technology for precision breeding applications: presentation of a case study in tomato.Frontiers in plant science · 2025Article
- Engineering tomato disease resistance by manipulating susceptibility genes.Frontiers in genome editing · 2025Review
- Insights ofFrontiers in plant science · 2025Article
16 more citing papers are in PubMed but not listed here.
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8 authors.
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Abstract
backgroundThe development of CRISPR/Cas9 technology has facilitated targeted mutagenesis in an efficient and precise way. Previously, RNAi silencing of the susceptibility (S) gene PowderyMildewResistance 4 (PMR4) in tomato has been shown to enhance resistance against the powdery mildew pathogen Oidium neolycopersici (On).
resultsTo study whether full knock-out of the tomato PMR4 gene would result in a higher level of resistance than in the RNAi-silenced transgenic plants we generated tomato PMR4 CRISPR mutants. We used a CRISPR/Cas9 construct containing four single-guide RNAs (sgRNAs) targeting the tomato PMR4 gene to increase the possibility of large deletions in the mutants. After PCR-based selection and sequencing of transformants, we identified five different mutation events, including deletions from 4 to 900-bp, a 1-bp insertion and a 892-bp inversion. These mutants all showed reduced susceptibility to On based on visual scoring of disease symptoms and quantification of relative fungal biomass. Histological observations revealed a significantly higher occurrence of hypersensitive response-like cell death at sites of fungal infection in the pmr4 mutants compared to wild-type plants. Both haustorial formation and hyphal growth were diminished but not completely inhibited in the mutants.
conclusionCRISPR/Cas-9 targeted mutagenesis of the tomato PMR4 gene resulted in mutants with reduced but not complete loss of susceptibility to the PM pathogen On. Our study demonstrates the efficiency and versatility of the CRISPR/Cas9 system as a powerful tool to study and characterize S-genes by generating different types of mutations.
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