ArticleBMC veterinary research2020
Development and application of a triplex real-time PCR assay for simultaneous detection of avian influenza virus, Newcastle disease virus, and duck Tembusu virus.
Article in BMC veterinary research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 28 citations in OpenAlex.
- A Systematic Review of New, Enhanced Surveillance Systems and Methodologies for Zoonotic Influenza Viruses in Animals and Human-Animal Interface.Influenza and other respiratory viruses · 2025Pooled it
- Development and Analytical Validation of a Multiplex Real-Time RT-PCR Assay for Simultaneous Detection of Avian Influenza A Virus and Newcastle Disease Virus.Pathogens (Basel, Switzerland) · 2026Article
- Development of a Triplex TaqMan Real-Time PCR Assay for Simultaneous Detection of Duck Hepatitis B Virus, Duck Adenovirus Type 3, andVeterinary sciences · 2026Article
- Pathogenesis of NGPV and DuCV CO-infection pathogenicity analysis of co-infection with novel goose parvovirus and duck circovirus in Cherry Valley meat ducks.Poultry science · 2026Article
- TaqMan-probe-based multiplex real-time qPCR assay for simultaneous detection of Duck Tembusu virus, Novel duck reovirus, and Duck circovirus.Frontiers in microbiology · 2026Article
- Development and application of a triplex real-time PCR method for the detection ofMicrobiology spectrum · 2025Article
- Evaluation of various filter paper and reagent systems for the preservation of Newcastle disease virus RNA samples.Journal of veterinary research · 2025Article
- Optimization and Validation of Universal Real-Time RT-PCR Assay to Detect Virulent Newcastle Disease Viruses.Viruses · 2025Article
- Establishment of one-step duplex TaqMan real-time PCR for detection of feline coronavirus and panleukopenia virus.Applied microbiology and biotechnology · 2025Article
- Development of a Rapid Visual Detection Assay for Duck Tembusu Virus Using RT-LAMP-CRISPR/Cas12a.Animals : an open access journal from MDPI · 2024Article
- One-Step Multiplex Real-Time Fluorescent Quantitative Reverse Transcription PCR for Simultaneous Detection of Four Waterfowl Viruses.Microorganisms · 2024Article
- Article
- Characterization and pathogenicity of a novel avian orthoreovirus in China.Frontiers in microbiology · 2024Article
- The use of RT-PCR in the diagnosis and differentiation of vaccine strains of chicken infectious bronchitis and Newcastle disease.Open veterinary journal · 2023Article
- Multiplex digital PCR: a superior technique to qPCR for the simultaneous detection of duck Tembusu virus, duck circovirus, and new duck reovirus.Frontiers in veterinary science · 2023Article
- Development of a reverse transcription loop-mediated isothermal amplification based clustered regularly interspaced short palindromic repeats Cas12a assay for duck Tembusu virus.Frontiers in microbiology · 2023Article
- Review detection of Newcastle disease virus.Frontiers in veterinary science · 2022Review
- Identification and Pathogenicity Analysis of the Pathogen Causing Spotted Spleen in Muscovy Duck.Frontiers in veterinary science · 2022Article
- New Insights into the Biology of the Emerging Tembusu Virus.Pathogens (Basel, Switzerland) · 2021Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundPathogens including duck-origin avian influenza virus (AIV), duck-origin Newcastle disease virus (NDV) and duck Tembusu virus (DTMUV) posed great harm to ducks and caused great economic losses to the duck industry. In this study, we aim to develop a triplex real-time polymerase chain reaction (PCR) assay to detect these three viruses as early as possible in the suspicious duck flocks.
resultsThe detection limit of the triplex real-time PCR for AIV, NDV, and DTMUV was 1 × 10
conclusionThe developed triplex real-time PCR is fast, specific and sensitive, and is feasible and effective for the simultaneous detection of AIV, NDV, and DTMUV in ducks.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.