Evidence map›Paper›PMID 32560308›Full record

ArticleMicromachines2020

3D Printed Monolithic Microreactors for Real-Time Detection of

Ole Behrmann, Matthias Hügle, Franz Eckardt, Iris Bachmann, Cecilia Heller, Marina Schramm, Carrie Turner, Frank T Hufert, Gregory Dame

Abstract read
In one paragraph

Article in Micromachines, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Ole BehrmannInstitute of Microbiology and Virology, Brandenburg Medical School Theodor Fontane, 16816 Neuruppin, Germany.ORCID 0000-0002-7846-7917
Matthias HügleInstitute of Microbiology and Virology, Brandenburg Medical School Theodor Fontane, 16816 Neuruppin, Germany.ORCID 0000-0003-0484-0245
Franz EckardtInstitute of Microbiology and Virology, Brandenburg Medical School Theodor Fontane, 16816 Neuruppin, Germany.
Iris BachmannInstitute of Microbiology and Virology, Brandenburg Medical School Theodor Fontane, 16816 Neuruppin, Germany.ORCID 0000-0001-9916-8230
Cecilia HellerInstitute of Microbiology and Virology, Brandenburg Medical School Theodor Fontane, 16816 Neuruppin, Germany.ORCID 0000-0001-6915-4823
Marina SchrammInstitute of Microbiology and Virology, Brandenburg Medical School Theodor Fontane, 16816 Neuruppin, Germany.
Carrie TurnerNational Infections Service, Public Health England, Porton Down SP4 0JG, UK.
Frank T HufertInstitute of Microbiology and Virology, Brandenburg Medical School Theodor Fontane, 16816 Neuruppin, Germany.
Gregory DameInstitute of Microbiology and Virology, Brandenburg Medical School Theodor Fontane, 16816 Neuruppin, Germany.

Funding

Brandenburger Staatsministerium für Wissenschaft, Forschung und Kultur 06-GeCa:H228-05/002/004Bundesministerium für Bildung und Forschung 16GW0145European Regional Development Fund 85017408
6 · The paper itself

Abstract

We investigate the compatibility of three 3D printing materials towards real-time recombinase polymerase amplification (rtRPA). Both the general ability of the rtRPA reaction to occur while in contact with the cured 3D printing materials as well as the residual autofluorescence and fluorescence drift in dependence on post curing of the materials is characterized. We 3D printed monolithic rtRPA microreactors and subjected the devices to different post curing protocols. Residual autofluorescence and drift, as well as rtRPA kinetics, were then measured in a custom-made mobile temperature-controlled fluorescence reader (mTFR). Furthermore, we investigated the effects of storage on the devices over a 30-day period. Finally, we present the single- and duplex rtRPA detection of both the organism-specific

Indexed as

3D printingantibiotic resistancerecombinase polymerase amplification (RPA)

Identifiers

PMID32560308
PMCPMC7344889

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.