ArticleNeuron2020
Direct Readout of Neural Stem Cell Transgenesis with an Integration-Coupled Gene Expression Switch.
Article in Neuron, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 42 citations in OpenAlex.
- Intensity modulation of trichromatic split fluorescent proteins for live cell mapping.Cell reports methods · 2026Article
- Molecular signatures of cell diversity modulated by long noncoding RNAs in the human fetal spinal cord.iScience · 2026Article
- Expanded iOn switch toolkit enables flexible clonal labeling and dynamic imaging in model and non-model animals.Communications biology · 2026Article
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- miRNA modules for precise, tunable control of gene expression.bioRxiv : the preprint server for biology · 2025Article
- Tbx genes influence early gene expression and photoreceptor patterning in the chick retina.bioRxiv : the preprint server for biology · 2025Article
- Transcriptomic Convergence and the Female Protective Effect in Autism.bioRxiv : the preprint server for biology · 2025Article
- Multicolor Cell Lineage Tracing Using MAGIC Markers Strategies.Methods in molecular biology (Clifton, N.J.) · 2025Article
- BEAM: A combinatorial recombinase toolbox for binary gene expression and mosaic genetic analysis.Cell reports · 2024Article
- Advanced Techniques Using In Vivo Electroporation to Study the Molecular Mechanisms of Cerebral Development Disorders.International journal of molecular sciences · 2023Review
- Cell Fate of Retinal Progenitor Cells: In Ovo UbC-StarTrack Analysis.International journal of molecular sciences · 2022Article
- Multicolor strategies for investigating clonal expansion and tissue plasticity.Cellular and molecular life sciences : CMLS · 2022Review
- Visualizing Cortical Development and Evolution: A Toolkit Update.Frontiers in neuroscience · 2022Review
- Potential of Multiscale Astrocyte Imaging for Revealing Mechanisms Underlying Neurodevelopmental Disorders.International journal of molecular sciences · 2021Review
- De novo ATP1A3 variants cause polymicrogyria.Science advances · 2021Article
- Deciphering neural heterogeneity through cell lineage tracing.Cellular and molecular life sciences : CMLS · 2021Review
- Unraveling the adult cell progeny of early postnatal progenitor cells.Scientific reports · 2020Article
- Stable integration of an optimized inducible promoter system enables spatiotemporal control of gene expression throughout avian development.Biology open · 2020Article
Corrections and comments
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Authors and funding
14 authors at 7 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stable genomic integration of exogenous transgenes is essential in neurodevelopmental and stem cell studies. Despite tools driving increasingly efficient genomic insertion with DNA vectors, transgenesis remains fundamentally hindered by the impossibility of distinguishing integrated from episomal transgenes. Here, we introduce an integration-coupled On genetic switch, iOn, which triggers gene expression upon incorporation into the host genome through transposition, thus enabling rapid and accurate identification of integration events following transfection with naked plasmids. In vitro, iOn permits rapid drug-free stable transgenesis of mouse and human pluripotent stem cells with multiple vectors. In vivo, we demonstrate faithful cell lineage tracing, assessment of regulatory elements, and mosaic analysis of gene function in somatic transgenesis experiments that reveal neural progenitor potentialities and interaction. These results establish iOn as a universally applicable strategy to accelerate and simplify genetic engineering in cultured systems and model organisms by conditioning transgene activation to genomic integration.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.