ArticleHepatology (Baltimore, Md.)2021
Intravital Dynamic and Correlative Imaging of Mouse Livers Reveals Diffusion-Dominated Canalicular and Flow-Augmented Ductular Bile Flux.
Article in Hepatology (Baltimore, Md.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 39 citations in OpenAlex.
- Article
- Synthesis, Characterization, and Potential Usefulness in Liver Function Assessment of Novel Bile Acid Derivatives with Near-Infrared Fluorescence (NIRBAD).Bioconjugate chemistry · 2024Article
- Intraocular liver spheroids for non-invasive high-resolution in vivo monitoring of liver cell function.Nature communications · 2024Article
- Basic concepts of mixture toxicity and relevance for risk evaluation and regulation.Archives of toxicology · 2023Article
- A modular analysis of bile canalicular function and its implications for cholestasis.American journal of physiology. Gastrointestinal and liver physiology · 2023Review
- Digital twin demonstrates significance of biomechanical growth control in liver regeneration after partial hepatectomy.iScience · 2023Article
- Article
- Inhibition of cytochrome P450 enhances the nephro- and hepatotoxicity of ochratoxin A.Archives of toxicology · 2022Article
- Colchicine overdose impairs the capacity of Kupffer cells to clear foreign particles and endotoxins.Archives of toxicology · 2022Article
- Hypoalbuminemia affects the spatio-temporal tissue distribution of ochratoxin A in liver and kidneys: consequences for organ toxicity.Archives of toxicology · 2022Article
- Compromised blood-bile barrier after acetaminophen overdose.Archives of toxicology · 2022Article
- Guided interactive image segmentation using machine learning and color-based image set clustering.Bioinformatics (Oxford, England) · 2022Article
- Transcription factor dynamics in plants: Insights and technologies for in vivo imaging.Plant physiology · 2022Review
- The role of albumin in compound transport: new possibilities by intravital imaging.EXCLI journal · 2022Article
- Liver specific, systemic and genetic contributors to alcohol-related liver disease progression.Zeitschrift fur Gastroenterologie · 2022Review
- Multiphoton intravital microscopy of rodents.Nature reviews. Methods primers · 2022Article
- Subcellular spatio-temporal intravital kinetics of aflatoxin BArchives of toxicology · 2021Article
- Highlight report: New insights in liver physiology: Canalicular bile flux is diffusion dominated.EXCLI journal · 2020Article
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Authors and funding
21 authors at 9 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
aimsSmall-molecule flux in tissue microdomains is essential for organ function, but knowledge of this process is scant due to the lack of suitable methods. We developed two independent techniques that allow the quantification of advection (flow) and diffusion in individual bile canaliculi and in interlobular bile ducts of intact livers in living mice, namely fluorescence loss after photoactivation and intravital arbitrary region image correlation spectroscopy. APPROACH AND
resultsThe results challenge the prevailing "mechano-osmotic" theory of canalicular bile flow. After active transport across hepatocyte membranes, bile acids are transported in the canaliculi primarily by diffusion. Only in the interlobular ducts is diffusion augmented by regulatable advection. Photoactivation of fluorescein bis-(5-carboxymethoxy-2-nitrobenzyl)-ether in entire lobules demonstrated the establishment of diffusive gradients in the bile canalicular network and the sink function of interlobular ducts. In contrast to the bile canalicular network, vectorial transport was detected and quantified in the mesh of interlobular bile ducts.
conclusionsThe liver consists of a diffusion-dominated canalicular domain, where hepatocytes secrete small molecules and generate a concentration gradient and a flow-augmented ductular domain, where regulated water influx creates unidirectional advection that augments the diffusive flux.
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