Evidence map›Paper›PMID 32556830›Full record

ArticleLasers in medical science2020

Laser Nd:YAG patterning enhance human osteoblast behavior on zirconia implants.

Beatriz Ferreira Fernandes, Mariana Brito da Cruz, Joana Faria Marques, Sara Madeira, Óscar Carvalho, Filipe Samuel Silva, António Duarte Sola Pereira da Mata, João Manuel Mendez Caramês

Abstract read
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In one paragraph

Article in Lasers in medical science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Article
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  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Beatriz Ferreira FernandesOral Biology and Biochemistry Research Group, LIBPhys, Faculty of Dental Medicine, Universidade de Lisboa, 1649-003, Lisboa, Portugal. beatriz-ferreira@campus.ul.pt.ORCID http://orcid.org/0000-0002-0334-4592
Mariana Brito da CruzOral Biology and Biochemistry Research Group, LIBPhys, Faculty of Dental Medicine, Universidade de Lisboa, 1649-003, Lisboa, Portugal.
Joana Faria MarquesOral Biology and Biochemistry Research Group, LIBPhys, Faculty of Dental Medicine, Universidade de Lisboa, 1649-003, Lisboa, Portugal.
Sara MadeiraCenter for Microelectromechanical Systems (CMEMS), Dept. of Mechanical Engineering, University of Minho, 4800-058, Guimarães, Portugal.
Óscar CarvalhoCenter for Microelectromechanical Systems (CMEMS), Dept. of Mechanical Engineering, University of Minho, 4800-058, Guimarães, Portugal.
Filipe Samuel SilvaCenter for Microelectromechanical Systems (CMEMS), Dept. of Mechanical Engineering, University of Minho, 4800-058, Guimarães, Portugal.
António Duarte Sola Pereira da MataOral Biology and Biochemistry Research Group, LIBPhys, Faculty of Dental Medicine, Universidade de Lisboa, 1649-003, Lisboa, Portugal.
João Manuel Mendez CaramêsBone Physiology Research Group, LIBPhys, Faculty of Dental Medicine, Universidade de Lisboa, 1649-003, Lisboa, Portugal.

Funding

Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa 01-0145-FEDER-030498
6 · The paper itself

Abstract

Zirconia has been regarded as a promising material for dental implants, and Nd:YAG laser treatment has been proposed as a potential strategy to improve its bioactivity. The main aim of the present study was to evaluate the in vitro behavior of human fetal osteoblasts in contact with laser-textured zirconia implant surfaces assessing the effect of different texture patterns, spacing between laser passes and number of laser passes. Zirconia discs were produced and treated with Nd:YAG laser according to test group variables: texture (microgrooves and micropillar array), distance between surface features (25 μm, 30 μm and 35 μm), and laser passes [1, 2, 4, and 8]. Untextured sandblasted and acid-etched zirconia discs (SBAE) were used as controls. Human osteoblasts (hFOB 1.19) were cultured for 14 days on test and control samples. Morphology and cellular adhesion were observed using scanning electron microscopy (SEM). Cell viability and proliferation were evaluated at 1, 3, 7, and 14 days using a commercial resazurin-based method. Collagen type I was evaluated at 3 days using ELISA. Alkaline phosphatase (ALP) activity was evaluated at 7 days using a colorimetric enzymatic technique. Group comparisons were tested using ANOVA or Mann-Whitney test (Tukey's post hoc) using statistical software, and significance was set at p < 0.05. Cell viability and proliferation increased over time for all groups with statistically higher values for laser-textured groups when compared with control at 7 and 14 days in culture (p < 0.05). Collagen type I levels were higher for study groups (p < 0.05) when compared with control group. No statistically differences were detected for ALP activity levels between texture and control groups (p > 0.05). The results suggest that laser-machined zirconia implant surfaces may benefit biological osteoblast response. However, the type of texture, spacing at the range of 25-35 μm, and number of laser passes did not seem to be relevant variables.

Indexed as

Lasers, Solid-StateProstheses and ImplantsCell AdhesionCells, CulturedCell ShapeCell SurvivalHumansOsteoblastsSurface PropertiesZirconiumZirconiumzirconium oxide“Dental implants”“Human osteoblasts”“Laser”“Zirconia”

Identifiers

PMID32556830

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.