ArticlePloS one2020
Ultrasound therapy with optimal intensity facilitates peripheral nerve regeneration in rats through suppression of pro-inflammatory and nerve growth inhibitor gene expression.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
- Low-Intensity Pulsed Ultrasound in Peripheral and Central Nerve Repair: Mechanisms and Emerging Therapeutic Strategies.Journal of functional biomaterials · 2026Review
- Peripheral nerve repair: innovations and future directions.Journal of translational medicine · 2026Review
- Low-intensity pulsed ultrasound for peripheral nerve regeneration: mechanobiological mechanisms and translational potential.Journal of translational medicine · 2025Review
- Facial nerve pathology: emerging strategies for regeneration and functional restoration.Journal of materials chemistry. B · 2025Review
- The Therapeutic Potential of Pulsed Electromagnetic Fields (PEMF) and Low-Intensity Pulsed Ultrasound (LIPUS) in Peripheral Nerve Regeneration: A Comprehensive Review.International journal of molecular sciences · 2025Review
- Efficacy of Manual Therapy and Electrophysical Modalities for Treatment of Cubital Tunnel Syndrome: A Randomized Interventional Trial.Life (Basel, Switzerland) · 2025Article
- Pathogenesis, Diagnosis, and Management of Trigeminal Neuralgia: A Narrative Review.Journal of clinical medicine · 2025Review
- Research progress in different physical therapies for treating peripheral nerve injuries.Frontiers in neurology · 2025Review
- Physical modulation and peripheral nerve regeneration: a literature review.Cell regeneration (London, England) · 2024Review
- Effects of PEMF and LIPUS Therapy on the Expression of Genes Related to Peripheral Nerve Regeneration in Schwann Cells.International journal of molecular sciences · 2024Article
- Advancements in stimulation therapies for peripheral nerve regeneration.Biomedical materials (Bristol, England) · 2024Review
- Combining external physical stimuli and nanostructured materials for upregulating pro-regenerative cellular pathways in peripheral nerve repair.Frontiers in cell and developmental biology · 2024Review
- Emerging role of extracellular vesicles and exogenous stimuli in molecular mechanisms of peripheral nerve regeneration.Frontiers in cellular neuroscience · 2024Review
- Innervation of an Ultrasound-Mediated PVDF-TrFE Scaffold for Skin-Tissue Engineering.Biomimetics (Basel, Switzerland) · 2023Article
- Ultrasound therapy for a week promotes regeneration and reduces pro-inflammatory macrophages in a rat sciatic nerve autograft model.Scientific reports · 2023Article
- Low-intensity low-frequency pulsed ultrasound ameliorates sciatic nerve dysfunction in a rat model of cisplatin-induced peripheral neuropathy.Scientific reports · 2022Article
- Ultrasound stimulation improves inflammatory resolution, neuroprotection, and functional recovery after spinal cord injury.Scientific reports · 2022Article
- Ultrasound Stimulation Inhibits Morphological Degeneration of Motor Endplates in the Denervated Skeletal Muscle of Rats.Neuroscience insights · 2022Article
- Therapeutic Low-Intensity Ultrasound for Peripheral Nerve Regeneration - A Schwann Cell Perspective.Frontiers in cellular neuroscience · 2021Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTherapeutic ultrasound (US) is a promising physical therapy modality for peripheral nerve regeneration. However, it is necessary to identify the most effective US parameters and clarify the underlying mechanisms before its clinical application. The intensity of US is one of the most important parameters. However, the optimum intensity for the promotion of peripheral nerve regeneration has yet to be determined.
objectivesTo identify the optimum intensity of US necessary for the promotion of peripheral nerve regeneration after crush injuries in rats and to clarify the underlying mechanisms of US by mRNA expression analysis.
methodsWe inflicted sciatic nerve crush injuries on adult Lewis rats and performed ultrasound irradiation using 4 different US intensities: 0 (sham stimulation), 30, 140, and 250 mW/cm2 with frequency (5 days/week) and duration (5 min/day). We evaluated peripheral nerve regeneration by quantitative real-time PCR one week after injury. Histomorphometric analyses and motor function analysis were evaluated 3 weeks after injury.
resultsUS stimulation enhanced re-myelination as well as sprouting of axons, especially at an intensity of 140 mW/cm2. mRNA expression revealed that US suppressed the expression of the inflammatory cytokines TNF and IL-6 and the axonal growth inhibitors SEMA3A and GSK3β.
conclusionsAn intensity of 140 mW/cm2 was optimal to support regeneration of the sciatic nerve after a crush injury in rats by, in part, the suppression of pro-inflammatory and nerve growth inhibitor gene expression.
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