Evidence map›Paper›PMID 32553916›Full record

ArticleActa biomaterialia2020

Spatially-directed cell migration in acoustically-responsive scaffolds through the controlled delivery of basic fibroblast growth factor.

Xiaofang Lu, Hai Jin, Carole Quesada, Easton C Farrell, Leidan Huang, Mitra Aliabouzar, Oliver D Kripfgans, J Brian Fowlkes, Renny T Franceschi, Andrew J Putnam and 1 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta biomaterialia, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
1.4field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 27 citations in OpenAlex.

  1. Review
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  8. Acoustically responsive scaffolds: Unraveling release kinetics and mechanisms for sustained, steady drug delivery.Journal of controlled release : official journal of the Controlled Release Society · 2024
    Article
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  10. The Role of Myofibroblasts in Physiological and Pathological Tissue Repair.Cold Spring Harbor perspectives in biology · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 2 countries.

Xiaofang LuDepartment of Radiology, University of Michigan, Ann Arbor, MI, USA.
Hai JinDepartment of Radiology, University of Michigan, Ann Arbor, MI, USA; School of Medicine, Second Affiliated Hospital of South China University of Technology, Guangzhou, China.
Carole QuesadaDepartment of Radiology, University of Michigan, Ann Arbor, MI, USA.
Easton C FarrellDepartment of Radiology, University of Michigan, Ann Arbor, MI, USA.
Leidan HuangDepartment of Radiology, University of Michigan, Ann Arbor, MI, USA; Department of Ultrasound, Second Affiliated Hospital of Army Medical University, Chongqing, China.
Mitra AliabouzarDepartment of Radiology, University of Michigan, Ann Arbor, MI, USA.
Oliver D KripfgansDepartment of Radiology, University of Michigan, Ann Arbor, MI, USA; Applied Physics Program, University of Michigan, Ann Arbor, MI, USA; Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA.
J Brian FowlkesDepartment of Radiology, University of Michigan, Ann Arbor, MI, USA; Applied Physics Program, University of Michigan, Ann Arbor, MI, USA; Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA.
Renny T FranceschiDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA; Dental School, University of Michigan, Ann Arbor, MI, USA.
Andrew J PutnamDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA.
Mario L FabiilliDepartment of Radiology, University of Michigan, Ann Arbor, MI, USA; Applied Physics Program, University of Michigan, Ann Arbor, MI, USA; Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA. Electronic address: mfabiill@umich.edu.
University of Michigan · USArmy Medical University · CN

Funding

Angiogenic growth factor delivery for vascular regeneration in critical limb ischemia using acoustically-responsive scaffoldsR01HL139656 · NHLBI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Mario Leonardo Fabiilli · 2018 to 2026
$5.7M
NHLBI NIH HHS R01 HL139656
6 · The paper itself

Abstract

Hydrogels are commonly used in regenerative medicine for the delivery of growth factors (GFs). The spatial and temporal presentations of GFs are critical for directing regenerative processes, yet conventional hydrogels do not enable such control. We have developed a composite hydrogel, termed an acoustically-responsive scaffold (ARS), where release of a GF is non-invasively and spatiotemporally-controlled using focused ultrasound. The ARS consists of a fibrin matrix doped with a GF-loaded, phase-shift emulsion. The GF is released when the ARS is exposed to suprathreshold ultrasound via a mechanism termed acoustic droplet vaporization. In this study, we investigate how different spatial patterns of suprathreshold ultrasound can impact the biological response upon in vivo implantation of an ARS containing basic fibroblast growth factor (bFGF). ARSs were fabricated with either perfluorohexane (bFGF-C6-ARS) or perflurooctane (bFGF-C8-ARS) within the phase-shift emulsion. Ultrasound generated stable bubbles in bFGF-C6-ARS, which inhibited matrix compaction, whereas transiently stable bubbles were generated in bFGF-C8-ARS, which decreased in height by 44% within one day of implantation. The rate of bFGF release and distance of host cell migration were up to 6.8-fold and 8.1-fold greater, respectively, in bFGF-C8-ARS versus bFGF-C6-ARS. Ultrasound increased the formation of macropores within the fibrin matrix of bFGF-C8-ARS by 2.7-fold. These results demonstrate that spatially patterning suprathreshold ultrasound within bFGF-C8-ARS can be used to elicit a spatially-directed response from the host. Overall, these findings can be used in developing strategies to spatially pattern regenerative processes. STATEMENT OF SIGNIFICANCE: Hydrogels are commonly used in regenerative medicine for the delivery of growth factors (GFs). The spatial and temporal presentations of GFs are critical for directing regenerative processes, yet conventional hydrogels do not enable such control. We have developed a composite hydrogel, termed an acoustically-responsive scaffold (ARS), where GF release is non-invasively and spatiotemporally-controlled using focused ultrasound. The ARS consists of a fibrin matrix doped with a phase-shift emulsion loaded with GF, which is released when the ARS is exposed to ultrasound. In this in vivo study, we demonstrate that spatially patterning ultrasound within an ARS containing basic fibroblast growth factor (bFGF) can elicit a spatially-directed response from the host. Overall, these findings can be used in developing strategies to spatially pattern regenerative processes.

Indexed as

Cell MovementFibrinFibroblast Growth Factor 2HydrogelsVolatilizationFibrinFibroblast Growth Factor 2HydrogelsAcoustic droplet vaporizationBasic fibroblast growth factorDrug deliveryFibrinMigrationPhase-shift emulsionUltrasound

Identifiers

PMID32553916
PMCPMC7423759
OpenAlexW3034888975

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.