Evidence map›Paper›PMID 32553552›Full record

ArticleComparative biochemistry and physiology. Part B, Biochemistry & molecular biology

N-glycosylation in Spodoptera frugiperda (Lepidoptera: Noctuidae) midgut membrane-bound glycoproteins.

Felipe Jun Fuzita, Kevin Brown Chandler, John R Haserick, Walter R Terra, Clélia Ferreira, Catherine E Costello

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 52% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Felipe Jun FuzitaCenter for Biomedical Mass Spectrometry, Department of Biochemistry, Boston University School of Medicine, Boston, MA, USA; Laboratory of Insect Biochemistry, Department of Biochemistry, University of São Paulo, São Paulo, Brazil. Electronic address: felipe.fuzita@usp.br.
Kevin Brown ChandlerCenter for Biomedical Mass Spectrometry, Department of Biochemistry, Boston University School of Medicine, Boston, MA, USA.
John R HaserickCenter for Biomedical Mass Spectrometry, Department of Biochemistry, Boston University School of Medicine, Boston, MA, USA.
Walter R TerraLaboratory of Insect Biochemistry, Department of Biochemistry, University of São Paulo, São Paulo, Brazil.
Clélia FerreiraLaboratory of Insect Biochemistry, Department of Biochemistry, University of São Paulo, São Paulo, Brazil.
Catherine E CostelloCenter for Biomedical Mass Spectrometry, Department of Biochemistry, Boston University School of Medicine, Boston, MA, USA.
Boston University · USUniversidade de São Paulo · BR

Funding

Mass Spectrometry Resource for Biology and MedicineP41GM104603 · NIGMS · BOSTON UNIVERSITY MEDICAL CAMPUS · PI COSTELLO, CATHERINE E. · 2012 to 2017
$10.4M
Legacy Support During Closure of the Mass Spectrometry Resource for Biology and MedicineR24GM134210 · NIGMS · BOSTON UNIVERSITY MEDICAL CAMPUS · PI COSTELLO, CATHERINE E. · 2019 to 2021
$2.1M
An open-source software suite for processing glycomics and glycoproteomics mass spectral dataU01CA221234 · NCI · BOSTON UNIVERSITY MEDICAL CAMPUS · PI ZAIA, JOSEPH · 2017 to 2019
$1.2M
MALDI-TOF/TOF MS TO SUPPORT BIOMEDICAL RESEARCHS10OD010724 · OD · BOSTON UNIVERSITY MEDICAL CAMPUS · PI COSTELLO, CATHERINE E. · 2012 to 2012
$590k
A Thermo-Fisher Scientific Q-Exactive HF Mass Spectrometry SystemS10OD021651 · OD · BOSTON UNIVERSITY MEDICAL CAMPUS · PI ZAIA, JOSEPH · 2016 to 2016
$580k
NCI NIH HHS U01 CA221234NIGMS NIH HHS P41 GM104603NIGMS NIH HHS R24 GM134210NIH HHS S10 OD010724NIH HHS S10 OD021651
6 · The paper itself

Abstract

Spodoptera frugiperda is a widely distributed agricultural pest. It has previously been established that glycoproteins in the midgut microvillar membrane of insects are targets for toxins produced by different organisms as well as plant lectins. However, there is still little information about the N-glycome of membrane-bound midgut glycoproteins in Lepidoptera and other insect groups. The present study used mass spectrometry-based approaches to characterize the N-glycoproteins present in the midgut cell microvilli of Spodoptera frugiperda. We subjected midgut cell microvilli proteins to proteolytic digestion and enriched the resulting glycopeptides prior to analysis. We also performed endoglycosidase release of N-glycans in the presence of H

Indexed as

AnimalsChromatography, LiquidDigestive SystemGlycosylationHydrolasesInsect ProteinsMass SpectrometryMembrane GlycoproteinsMicrovilliProteomeProteomicsSpodopteraTransferrinHydrolasesInsect ProteinsMembrane GlycoproteinsProteomeTransferrinAminopeptidase NGlycoproteinsGlycoproteomicsMidgut microvillar membraneSpodoptera frugiperdaTransferrin

Identifiers

PMID32553552
PMCPMC8130577
OpenAlexW3034375662

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.