Evidence map›Paper›PMID 32552416›Full record

ArticleJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc2020

Development of a droplet digital PCR for detection and quantification of porcine epidemic diarrhea virus.

Wei W Cao, Dong S He, Zhen J Chen, Yu Z Zuo, Xun Chen, Yan L Chang, Zhi G Zhang, Lei Ye, Lei Shi

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.2field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 11 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Wei W CaoInstitute of Food Safety and Nutrition, Jinan University, Guangzhou, China (Cao, Z.J. Chen, X. Chen, Chang, Shi, Ye); College of Veterinary Medicine, South China Agricultural University, Guangzhou, China (He); College of Animal Science and Technology, Agricultural University of Hebei, Baoding, China (Zuo); State Key Laboratory of Food Safety Technology for Meat Products and Synergetic Innovation Center of Food Safety and Nutrition, Xiamen Yinxiang Group, Xiamen, China (Zhang, Shi).
Dong S HeInstitute of Food Safety and Nutrition, Jinan University, Guangzhou, China (Cao, Z.J. Chen, X. Chen, Chang, Shi, Ye); College of Veterinary Medicine, South China Agricultural University, Guangzhou, China (He); College of Animal Science and Technology, Agricultural University of Hebei, Baoding, China (Zuo); State Key Laboratory of Food Safety Technology for Meat Products and Synergetic Innovation Center of Food Safety and Nutrition, Xiamen Yinxiang Group, Xiamen, China (Zhang, Shi).
Zhen J ChenInstitute of Food Safety and Nutrition, Jinan University, Guangzhou, China (Cao, Z.J. Chen, X. Chen, Chang, Shi, Ye); College of Veterinary Medicine, South China Agricultural University, Guangzhou, China (He); College of Animal Science and Technology, Agricultural University of Hebei, Baoding, China (Zuo); State Key Laboratory of Food Safety Technology for Meat Products and Synergetic Innovation Center of Food Safety and Nutrition, Xiamen Yinxiang Group, Xiamen, China (Zhang, Shi).
Yu Z ZuoInstitute of Food Safety and Nutrition, Jinan University, Guangzhou, China (Cao, Z.J. Chen, X. Chen, Chang, Shi, Ye); College of Veterinary Medicine, South China Agricultural University, Guangzhou, China (He); College of Animal Science and Technology, Agricultural University of Hebei, Baoding, China (Zuo); State Key Laboratory of Food Safety Technology for Meat Products and Synergetic Innovation Center of Food Safety and Nutrition, Xiamen Yinxiang Group, Xiamen, China (Zhang, Shi).
Xun ChenInstitute of Food Safety and Nutrition, Jinan University, Guangzhou, China (Cao, Z.J. Chen, X. Chen, Chang, Shi, Ye); College of Veterinary Medicine, South China Agricultural University, Guangzhou, China (He); College of Animal Science and Technology, Agricultural University of Hebei, Baoding, China (Zuo); State Key Laboratory of Food Safety Technology for Meat Products and Synergetic Innovation Center of Food Safety and Nutrition, Xiamen Yinxiang Group, Xiamen, China (Zhang, Shi).
Yan L ChangInstitute of Food Safety and Nutrition, Jinan University, Guangzhou, China (Cao, Z.J. Chen, X. Chen, Chang, Shi, Ye); College of Veterinary Medicine, South China Agricultural University, Guangzhou, China (He); College of Animal Science and Technology, Agricultural University of Hebei, Baoding, China (Zuo); State Key Laboratory of Food Safety Technology for Meat Products and Synergetic Innovation Center of Food Safety and Nutrition, Xiamen Yinxiang Group, Xiamen, China (Zhang, Shi).
Zhi G ZhangInstitute of Food Safety and Nutrition, Jinan University, Guangzhou, China (Cao, Z.J. Chen, X. Chen, Chang, Shi, Ye); College of Veterinary Medicine, South China Agricultural University, Guangzhou, China (He); College of Animal Science and Technology, Agricultural University of Hebei, Baoding, China (Zuo); State Key Laboratory of Food Safety Technology for Meat Products and Synergetic Innovation Center of Food Safety and Nutrition, Xiamen Yinxiang Group, Xiamen, China (Zhang, Shi).
Lei YeInstitute of Food Safety and Nutrition, Jinan University, Guangzhou, China (Cao, Z.J. Chen, X. Chen, Chang, Shi, Ye); College of Veterinary Medicine, South China Agricultural University, Guangzhou, China (He); College of Animal Science and Technology, Agricultural University of Hebei, Baoding, China (Zuo); State Key Laboratory of Food Safety Technology for Meat Products and Synergetic Innovation Center of Food Safety and Nutrition, Xiamen Yinxiang Group, Xiamen, China (Zhang, Shi).ORCID https://orcid.org/0000-0003-1696-3701
Lei ShiInstitute of Food Safety and Nutrition, Jinan University, Guangzhou, China (Cao, Z.J. Chen, X. Chen, Chang, Shi, Ye); College of Veterinary Medicine, South China Agricultural University, Guangzhou, China (He); College of Animal Science and Technology, Agricultural University of Hebei, Baoding, China (Zuo); State Key Laboratory of Food Safety Technology for Meat Products and Synergetic Innovation Center of Food Safety and Nutrition, Xiamen Yinxiang Group, Xiamen, China (Zhang, Shi).
South China Agricultural University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Porcine epidemic diarrhea, a disease caused by porcine epidemic diarrhea virus (PEDV), results in large economic losses to the global swine industry. To manage this disease effectively, it is essential to detect PEDV early and accurately. We developed a sensitive and accurate droplet digital PCR (ddPCR) assay to detect PEDV. The optimal primer-to-probe concentration and melting temperature were identified as 300:200 nM and 59.2°C, respectively. The specificity of the ddPCR assay was confirmed by negative test results for common swine pathogens. The detection limit for the ddPCR was 0.26 copies/μL, which is a 5.7-fold increase in sensitivity compared to that of real-time PCR (rtPCR). Both ddPCR and rtPCR assays exhibited good linearity, although ddPCR provided higher sensitivity for clinical detection compared to that of rtPCR. Our ddPCR methodology provides a promising tool for evaluating the PEDV viral load when used for clinical testing, particularly for detecting samples with low-copy viral loads.

Indexed as

AnimalsCoronavirus InfectionsPolymerase Chain ReactionPorcine epidemic diarrhea virusSensitivity and SpecificitySus scrofaSwineSwine DiseasesViral Loaddetectiondroplet digital PCRporcine epidemic diarrhea virusquantification

Identifiers

PMID32552416
PMCPMC7438641
OpenAlexW3036783164

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.