Evidence map›Paper›PMID 32551379›Full record

ArticleHeliyon2020

Establishment and transcriptomic analyses of a cattle rumen epithelial primary cells (REPC) culture by bulk and single-cell RNA sequencing to elucidate interactions of butyrate and rumen development.

Shudai Lin, Lingzhao Fang, Xiaolong Kang, Shuli Liu, Mei Liu, Erin E Connor, Ransom L Baldwin, George Liu, Cong-Jun Li

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Heliyon, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
2.3field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 19 citations in OpenAlex.

  1. Effects ofAnimals : an open access journal from MDPI · 2026
    Article
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  8. Advances in single-cell transcriptomics in animal research.Journal of animal science and biotechnology · 2024
    Review
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  10. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 3 countries.

Shudai LinAnimal Genomics and Improvement Laboratory, Agricultural Research Service, USDA, Beltsville, MD, USA.
Lingzhao FangAnimal Genomics and Improvement Laboratory, Agricultural Research Service, USDA, Beltsville, MD, USA.
Xiaolong KangAnimal Genomics and Improvement Laboratory, Agricultural Research Service, USDA, Beltsville, MD, USA.
Shuli LiuAnimal Genomics and Improvement Laboratory, Agricultural Research Service, USDA, Beltsville, MD, USA.
Mei LiuAnimal Genomics and Improvement Laboratory, Agricultural Research Service, USDA, Beltsville, MD, USA.
Erin E ConnorAnimal Genomics and Improvement Laboratory, Agricultural Research Service, USDA, Beltsville, MD, USA.
Ransom L BaldwinAnimal Genomics and Improvement Laboratory, Agricultural Research Service, USDA, Beltsville, MD, USA.
George LiuAnimal Genomics and Improvement Laboratory, Agricultural Research Service, USDA, Beltsville, MD, USA.
Cong-Jun LiAnimal Genomics and Improvement Laboratory, Agricultural Research Service, USDA, Beltsville, MD, USA.
Agricultural Research Service · USSouth China Agricultural University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

As a critical and high-value tool to study the development of rumen, we established a stable rumen epithelial primary cell (REPC) culture from a two-week-old Holstein bull calf rumen epithelial tissue. The transcriptomic profiling of the REPC and the direct effects of butyrate on gene expression were assessed. Correlated gene networks elucidated the putative roles and mechanisms of butyrate action in rumen epithelial development. The top networks perturbed by butyrate were associated with epithelial tissue development. Additionally, two critical upstream regulators, E2F1 and TGFB1, were identified to play critical roles in the differentiation, development, and growth of epithelial cells. Significant expression changes of upstream regulators and transcription factors provided further evidence in support that butyrate plays a specific and central role in regulating genomic and epigenomic activities influencing rumen development. This work is the essential component to obtain a complete global landscape of regulatory elements in cattle and to explore the dynamics of chromatin states in rumen epithelial cells induced by butyrate at early developmental stages.

Indexed as

AgricultureBioinformaticsButyrateCell biologyDevelopmental biologyEpigenetic regulationEpitheliumRumenTranscriptome

Identifiers

PMID32551379
PMCPMC7287249
OpenAlexW3034563078

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.