Evidence map›Paper›PMID 32550544›Full record

ArticleGene: X2019

Development of a high efficient promoter finding method based on transient transfection.

Yao Lu, Qilong Li, Kexin Zheng, Chenghao Fu, Chunying Jiang, Dayu Zhou, Chao Xia, Shiliang Ma

Open access · hybridAbstract read
In one paragraph

Article in Gene: X, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 49% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Yao LuCollege of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, Liaoning, PR China.
Qilong LiCollege of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, Liaoning, PR China.
Kexin ZhengCollege of Food Science and Technology, Shenyang Agricultural University, Shenyang, Liaoning, PR China.
Chenghao FuCollege of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, Liaoning, PR China.
Chunying JiangCollege of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, Liaoning, PR China.
Dayu ZhouCollege of Food Science and Technology, Shenyang Agricultural University, Shenyang, Liaoning, PR China.
Chao XiaCollege of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, Liaoning, PR China.
Shiliang MaCollege of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, Liaoning, PR China.
Shenyang Agricultural University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In metazoan genome, the mechanism of gene expression regulation between transcriptional regulatory elements and their target gene is spatiotemporal. Active promoters possess many specific chromosomal features, such as hypersensitive to DNaseI and enrichment of specific histone modifications. In this article, we proposed a novel method which possesses a high efficiency to find promoters

Indexed as

ATAC-seq, Assay for transposase-accessible chromatin using sequencingBioinformaticsCAGE, cap analysis of gene expressionCancer-specific promoterChIP-seq, Chromatin immunoprecipitation followed by massively parallel DNA sequencingCMV, CytomegalovirusCt, thresholdDHS, DNaseI hypersensitive sitesDNase-seq, DNase I hypersensitive sites sequencingdNTPs, deoxy-ribonucleoside triphosphateEF1a1, eukaryotic translation elongation factor 1 alpha 1eRNAs, enhancer RNAsFBS, fetal bovine serumGene expression regulationGene findingGRO-seq, global run-on sequencingH3K4me3, histone H3 lysine 4 trimethylationItpr2, inositol 1, 4, 5-triphosphate receptor 2LSINCT5, long stress-induced non-coding transcript 5MCS, multiple cloning siteMouse breast cancerMPRA, Massively parallel reporter assaysmSEAP, mouse synthetic secreted embryonic alkaline phosphatasePBS, phosphate buffered solutionpNPP, p-nitropheny-phosatePromoter trapqRT-PCR, quantitative RT-PCRRNA-seq, RNA sequencingSD, standard deviationSTARR-seq, Self-transcribing active regulatory region sequencingTFs, transcription factorstpk1, thiamine pyrophosphokinaseTSS, transcription start sites

Identifiers

PMID32550544
PMCPMC7286058
OpenAlexW2911302813

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.