ArticlePlant methods2020
A detached leaf assay for testing transient gene expression and gene editing in cowpea (
Article in Plant methods, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 54 citations in OpenAlex.
- A FnWRKY17-Frontiers in plant science · 2026Article
- Advances in gene editing for legume improvement: technologies, progress, and prospects.Frontiers in genome editing · 2026Review
- A panomics-driven framework for the improvement of major food legume crops: advances, challenges, and future prospects.Horticulture research · 2025Article
- Combining the CowPEAsy Web Application With in Planta Agroinfiltration for Native Promoter Validation in Vigna unguiculata.Plant, cell & environment · 2025Article
- Establishing a CRISPR/Cas9 genome editing framework in pigeonpea (Cajanus cajan L.) by targeting phytoene desaturase (PDS) gene disruption.Journal, genetic engineering & biotechnology · 2025Article
- Lupins in the genome editing era: advances in plant cell culture, double haploid technology and genetic transformation for crop improvement.Frontiers in plant science · 2025Review
- Genome editing in grain legumes for food security.Frontiers in genome editing · 2025Review
- CtMYB1 regulates flavonoid biosynthesis in safflower flower by binding the CAACCA elements.PloS one · 2025Article
- Comparative Evaluation of Transient Protein Expression Efficiency in Tissues across Soybean Varieties Using the Tsukuba System.Plants (Basel, Switzerland) · 2024Article
- Crop Landraces and Indigenous Varieties: A Valuable Source of Genes for Plant Breeding.Plants (Basel, Switzerland) · 2024Review
- The Histone Variant H3.3 Is Required for Plant Growth and Fertility inInternational journal of molecular sciences · 2024Article
- The establishment of transient expression systems and their application for gene function analysis of flavonoid biosynthesis in Carthamus tinctorius L.BMC plant biology · 2023Article
- Clustered regularly interspaced short palindromic repeats/CRISPR-associated protein and hairy roots: a perfect match for gene functional analysis and crop improvement.Current opinion in biotechnology · 2023Review
- Recalcitrance to transformation, a hindrance for genome editing of legumes.Frontiers in genome editing · 2023Review
- Nutritional, functional, and bioactive properties of african underutilized legumes.Frontiers in plant science · 2023Review
- Major viral diseases in grain legumes: designing disease resistant legumes from plant breeding and OMICS integration.Frontiers in plant science · 2023Review
- Article
- Cowpea speed breeding using regulated growth chamber conditions and seeds of oven-dried immature pods potentially accommodates eight generations per year.Plant methods · 2022Article
- Functional Allele Validation by Gene Editing to Leverage the Wealth of Genetic Resources for Crop Improvement.International journal of molecular sciences · 2022Review
- RNA Pol III promoters-key players in precisely targeted plant genome editing.Frontiers in genetics · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe legume cowpea (
resultsAttempts to develop a transient protoplast system for cowpea were unsuccessful. Leaflets from plants 3-4 weeks post-germination were age selected to establish a rapid
conclusionThe transient, detached cowpea leaf assay, and supporting analytical methods developed, provide a rapid and reproducible means for testing gene expression constructs, and constructs for inducing mutagenesis in genes involved in both vegetative and reproductive developmental programs. The method and tested editing constructs and components have potential application for a range of crop legumes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.