Evidence map›Paper›PMID 32548820›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2020

Live-Cell FRET Imaging of Phosphorylation-Dependent Caveolin-1 Switch.

Adriana M Zimnicka, Zhenlong Chen, Peter T Toth, Richard D Minshall

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Adriana M ZimnickaDepartment of Pharmacology, University of Illinois at Chicago, Chicago, IL, USA.
Zhenlong ChenDepartment of and Anesthesiology, University of Illinois at Chicago, Chicago, IL, USA.
Peter T TothDepartment of Pharmacology, University of Illinois at Chicago, Chicago, IL, USA.
Richard D MinshallDepartment of Pharmacology, University of Illinois at Chicago, Chicago, IL, USA. rminsh@uic.edu.

Funding

TRPC6 Regulation of Lung Endothelial Barrier FunctionP01HL060678 · NHLBI · UNIVERSITY OF ILLINOIS AT CHICAGO · PI MALIK, ASRAR B. · 2000 to 2020
$40.1M
Src Regulation of Lung Endothelial Barrier FunctionR01HL071626 · NHLBI · UNIVERSITY OF ILLINOIS AT CHICAGO · PI MINSHALL, RICHARD D · 2003 to 2011
$2.4M
Zeiss Dynamic Laser TIRFS10RR022547 · NCRR · UNIVERSITY OF ILLINOIS AT CHICAGO · PI MINSHALL, RICHARD D · 2007 to 2007
$225k
NCRR NIH HHS S10 RR022547NHLBI NIH HHS P01 HL060678NHLBI NIH HHS R01 HL071626
6 · The paper itself

Abstract

The detection of dynamic conformational changes in proteins in live cells is challenging. Live-cell FRET (Förster Resonance Energy Transfer) is an example of a noninvasive technique that can be used to achieve this goal at nanometer resolution. FRET-based assays are dependent on the presence of fluorescent probes, such as CFP- and YFP-conjugated protein pairs. Here, we describe an experimental protocol in which live-cell FRET was used to measure conformational changes in caveolin-1 (Cav-1) oligomers on the surface of plasmalemma vesicles, or caveolae.

Indexed as

CaveolaeCaveolin 1Fluorescence Resonance Energy TransferHEK293 CellsHumansImage Processing, Computer-AssistedLuminescent ProteinsPhosphorylationTransfectionCaveolin 1Luminescent ProteinsCav-1-CFPCav-1-YFPCaveolaeConformation changeLive-cell FRET

Identifiers

PMID32548820
PMCPMC9828887

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.