Evidence map›Paper›PMID 32548656›Full record

ArticleStem cell reviews and reports2020

Side-Population Trophoblasts Exhibit the Differentiation Potential of a Trophoblast Stem Cell Population, Persist to Term, and are Reduced in Fetal Growth Restriction.

Teena Kjb Gamage, Jasper J Perry, Vicky Fan, Katie Groom, Lawrence W Chamley, Joanna L James

Abstract read
PubMed Publisher
In one paragraph

Article in Stem cell reviews and reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. The Endometrial Stem/Progenitor Cells and Their Niches.Stem cell reviews and reports · 2024
    Review
  5. Article
  6. Review
  7. Observational
  8. Review
  9. Article
  10. Review
  11. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Teena Kjb GamageDepartment of Obstetrics and Gynaecology, University of Auckland, 85 Park Rd, Grafton Auckland, 1023, New Zealand.
Jasper J PerryBioinformatics Institute, University of Auckland, Auckland, 1023, New Zealand.
Vicky FanBioinformatics Institute, University of Auckland, Auckland, 1023, New Zealand.
Katie GroomThe Liggins Institute, University of Auckland, Auckland, 1023, New Zealand.
Lawrence W ChamleyDepartment of Obstetrics and Gynaecology, University of Auckland, 85 Park Rd, Grafton Auckland, 1023, New Zealand.
Joanna L JamesDepartment of Obstetrics and Gynaecology, University of Auckland, 85 Park Rd, Grafton Auckland, 1023, New Zealand. j.james@auckland.ac.nz.ORCID 0000-0002-3757-4177

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundFetal growth restriction often results from poor placental function and is a major cause of stillbirth. Clinically, fetal growth restriction is difficult to diagnose and currently has no effective treatment. Trophoblasts are unique placental cells that form the feto-maternal interface and facilitate nutrient and gas exchange. Fetal growth restriction is linked to inadequate trophoblast function. However, our understanding of the mechanisms underlying this dysfunction are poor, in part because of our inability to isolate and study the trophoblast stem cells from which mature trophoblasts arise in pathologic pregnancies.

methodsCells isolated from first-trimester placentae using the Hoechst side-population technique were propagated or differentiated into mature trophoblasts. Side-population trophoblasts were isolated from normal third-trimester and growth restricted placentae using the same technique. First and third-trimester side-population trophoblasts were compared by microarray analysis.

resultsFirst-trimester side-population trophoblasts could be propagated in an undifferentiated state or differentiated, via intermediate cytotrophoblasts, into syncytiotrophoblast or extravillous trophoblasts. Using the same technique, side-population trophoblasts could be isolated from term placentae for the first time, demonstrating that while they were present at consistent levels throughout gestation (~3·5%), side-population trophoblasts were significantly depleted in growth restricted pregnancies (0·32%).

conclusionsOur novel method of isolating a population of human trophoblast stem cell-like cells directly from human placental tissue throughout gestation provides the first insights into trophoblast dysfunction in pregnancy pathologies. The depletion of side-population trophoblasts in growth restricted placentae may contribute to poor placental function.

Indexed as

Cell DifferentiationAdultFemaleFetal Growth RetardationFetusHumansPlacentaPregnancyPregnancy Trimester, FirstPregnancy Trimester, ThirdStem CellsTrophoblastsFetal growth restrictionHuman trophoblast stem cellPlacentaPregnancy

Identifiers

PMID32548656

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.