Evidence map›Paper›PMID 32547868›Full record

ArticlePeerJ2020

The transferability of microsatellite loci from a homoploid to a polyploid hybrid complex: an example from fine-leaved

Przemysław P Tomczyk, Marcin Kiedrzyński, Iwona Jedrzejczyk, Monika Rewers, Pawel Wasowicz

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PubMed Full text at PMC Publisher
In one paragraph

Article in PeerJ, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Przemysław P TomczykDepartment of Geobotany and Plant Ecology, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland.
Marcin KiedrzyńskiDepartment of Geobotany and Plant Ecology, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland.
Iwona JedrzejczykLaboratory of Molecular Biology and Cytometry, Department of Agricultural Biotechnology, UTP University of Science and Technology, Bydgoszcz, Poland.
Monika RewersLaboratory of Molecular Biology and Cytometry, Department of Agricultural Biotechnology, UTP University of Science and Technology, Bydgoszcz, Poland.
Pawel WasowiczIcelandic Institute of Natural History, Akureyri, Iceland.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMicrosatellite loci, or single sequence repeats (SSR), are widely used as powerful markers in population genetics. They represent an attractive tool for studying plants such as grasses, whose evolution is driven by hybridisation and polyploidization. However, the development of microsatellite markers has been challenging and time-consuming, especially for non-model organisms lacking available genome-wide sequence data. One straightforward and low-cost approach is to transfer the SSR loci developed for one species, or complex, to another closely-related one. This work evaluates the transferability of microsatellite loci from homoploid to allopolyploid complexes of fine-leaved

methodsThe ploidy level within populations was estimated using flow cytometry. One diploid and one tetraploid population of

resultsFlow cytometry confirmed earlier data regarding DNA content in the investigated species and cytotypes. The phylogenetic ITS tree indicated a small genetic distance between

conclusionsThe transferred markers are valid tools for the study of population genetics and inheritance relationships within cytotypes and species and between them. The presented markers can be used to study inbreeding depression in the

Indexed as

Endemic speciesGenetic diversityGrassesLoliinaeMolecular ecologyPolyploidyPooideaePopulation geneticsSSR

Identifiers

PMID32547868
PMCPMC7271882

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.