ArticlePeerJ2020
Developments in marine invertebrate primary culture reveal novel cell morphologies in the model bivalve
Article in PeerJ, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 46 citations in OpenAlex.
- Development of long-term oyster explant cultures enable sustained tissue-specific activity and reveal novel cellular behaviours.Biochemistry and biophysics reports · 2026Article
- AdvancingBiology · 2026Article
- Advancing marine invertebrate cell line research: four key knowledge gaps.In vitro cellular & developmental biology. Animal · 2025Review
- Mechanisms of HAHV-1 Interaction with Hemocytes inBiology · 2025Article
- Ex Vivo Propagation of Pinctada Birnavirus Using Mantle Tissue Fragment Culture: Application for Measuring Replication at Different Temperatures, TCIDPathogens (Basel, Switzerland) · 2025Article
- Laboratory Replication of Ostreid Herpes Virus (OsHV-1) Using Pacific Oyster Tissue Explants.Viruses · 2024Article
- Cell-cultivated aquatic food products: emerging production systems for seafood.Journal of biological engineering · 2024Review
- Review
- A noveliScience · 2024Article
- A Straightforward Method for the Isolation and Cultivation ofInternational journal of molecular sciences · 2022Article
- Status in molluscan cell line development in last one decade (2010-2020): impediments and way forward.Cytotechnology · 2022Review
- Biomaterials and Bioactive Natural Products from Marine Invertebrates: From Basic Research to Innovative Applications.Marine drugs · 2022Review
- Cell culture and genetic transfection methods for the Japanese scallop, Patinopecten yessoensis.FEBS open bio · 2021Article
- Potential of genomic technologies to improve disease resistance in molluscan aquaculture.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2021Review
- Establishment of primary cell culture of Ruditapes decussatus haemocytes for metal toxicity assessment.In vitro cellular & developmental biology. Animal · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Cell culture provides useful model systems used in a wide range of biological applications, but its utility in marine invertebrates is limited due to the lack of immortalised cell lines. Primary cell and tissue cultures are typically used but remain poorly characterised for oysters, which can cause issues with experimental consistency and reproducibility. Improvements to methods of repeatable isolation, culture, and characterisation of oyster cells and tissues are required to help address these issues. In the current study, systematic improvements have been developed to facilitate the culture of primary cells from adult Pacific oyster tissues and identify novel cell morphologies that have not been reported previously. Cultures analysed by light microscopy, qPCR, and live cell imaging demonstrated maintenance of live, metabolically active Pacific oyster cells for several weeks post-explant. Interestingly, whole hearts dissected from adult oysters were found to continue contracting rhythmically up to 8 weeks after being transferred to a tissue culture system. Mantle tissue explants were also actively moving in the culture system. These improvements in primary cell culture of bivalves may be beneficial for research in ecotoxicology, virology, immunology, and genetic resistance to disease.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.