Evidence map›Paper›PMID 32547637›Full record

ArticleTherapeutic advances in gastroenterology2020

Determination of distinctive hypomethylated genes in African American colorectal neoplastic lesions.

Hassan Ashktorab, Kareem Washington, Shatha Zarnogi, Afnan Shakoori, Sudhir Varma, Edward Lee, Babak Shokrani, Adeyinka Laiyemo, Hassan Brim

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Therapeutic advances in gastroenterology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 9 citations in OpenAlex.

  1. Dynamic Impacts of Stock Enhancement on Kaluga Sturgeon (International journal of molecular sciences · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Hassan AshktorabDepartment of Pathology, and Medicine, Howard University, 2041 Georgia Avenue NW, Washington, DC 20059, USA.
Kareem WashingtonDepartment of Genetics, Howard University Hospital, Washington, DC, USA.
Shatha ZarnogiDepartment of Genetics, Howard University Hospital, Washington, DC, USA.
Afnan ShakooriDepartment of Genetics, Howard University Hospital, Washington, DC, USA.
Sudhir VarmaHithru Analytics, Laurel, MD, USA.
Edward LeeDepartment of Pathology, Howard University Hospital, Washington, DC, USA.
Babak ShokraniDepartment of Pathology, Howard University Hospital, Washington, DC, USA.
Adeyinka LaiyemoDepartment of Medicine, Howard University Hospital, Washington, DC, USA.
Hassan BrimDepartment of Pathology, and Medicine, Howard University, 2041 Georgia Avenue NW, Washington, DC 20059, USA.ORCID https://orcid.org/0000-0002-3361-9243
Howard University Hospital · USHoward University · US

Funding

Vanderbilt-Ingram Cancer Center SPORE in Gastrointestinal CancerP50CA236733 · NCI · VANDERBILT UNIVERSITY MEDICAL CENTER · PI MARY Kay WASHINGTON · 2019 to 2026
$19.6M
PROTEOMICS CORE FACILITYG12MD007597 · NIMHD · HOWARD UNIVERSITY · PI SOUTHERLAND, WILLIAM M. · 2012 to 2018
$14.8M
NCI NIH HHS P50 CA236733NIMHD NIH HHS G12 MD007597
6 · The paper itself

Abstract

backgroundFew studies have analyzed progressive demethylation in the path to cancer. This is of utmost importance, especially in populations such as African Americans, who display aggressive tumors at diagnosis, and for whom markers of early neoplastic transformation are needed. Here, we determined hypomethylated targets in the path to colorectal cancer (CRC) using Reduced Representation Bisulfite Sequencing (RRBS).

methodsDNA was extracted from fresh frozen tissues of patients with different colon lesions (normal, tubular adenoma, tubulovillous adenoma, and five cancers). RRBS was performed on these DNA extracts to identify hypomethylated gene targets. Alignment, mapping, and methylation analyses were performed. Pathways affected by the hypomethylated gene targets were determined using Ingenuity Pathway Analysis (IPA).

resultsPairwise analyses of samples led to the identification of the following novel hypomethylated genes:

conclusionsThis work provides insight into the role of hypomethylation in colon carcinogenesis in African Americans. The identified targets affected many important pathways, as demonstrated through IPA. These targets might serve as biomarkers for early diagnosis and potential targets for therapy.

Indexed as

African Americanscolorectal cancerDNA methylationhypomethylationRRBS

Identifiers

PMID32547637
PMCPMC7273615
OpenAlexW3031253912

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.