ArticleFrontiers in physiology2020
Ovarian Innervation Coupling With Vascularity: The Role of Electro-Acupuncture in Follicular Maturation in a Rat Model of Polycystic Ovary Syndrome.
Article in Frontiers in physiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 2 of them syntheses that pooled it.
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Who cites it
16 citing papers in PubMed, 2 syntheses or guidelines pooled it, 27 citations in OpenAlex.
- Efficacy of Acupuncture in Alleviating Negative Emotions in Polycystic Ovary Syndrome: A Systematic Review and Meta-Analysis.Brain and behavior · 2025Pooled it
- Clinical evidence of acupuncture for luteinized unruptured follicle syndrome: a systematic review and meta-analysis of randomized controlled trials.Frontiers in endocrinology · 2025Pooled it
- Real-Time and High-Resolution NIR-II-L Imaging of Netrin-1-Mediated Neurovascular Coupling Driven by Dose-Dependent Electroacupuncture.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Three-dimensional visualisation of human ovarian follicles using a whole-mount immunolabelling and optical tissue clearing method.Reproduction & fertility · 2025Article
- Distinct mechanisms of electroacupuncture and manual acupuncture in modulating hypothalamic GnRH-tanycyte unit function of polycystic ovary syndrome.Chinese medicine · 2025Article
- Regulating Integrin β1 to Restore Gonadotropin-Releasing Hormone-Tanycyte Unit Function in Polycystic Ovary Syndrome-Related Hypothalamic Dysregulation.Research (Washington, D.C.) · 2025Article
- Interstitial fluid transport in linea alba is involved in acupuncture-induced attenuation of ovarian hypofunction in aged rats.Frontiers in endocrinology · 2025Article
- 3D visualization of uterus and ovary: tissue clearing techniques and biomedical applications.Frontiers in bioengineering and biotechnology · 2025Review
- 3D visualization of cellular and molecular distributions in human crystalline lenses at different ages.American journal of translational research · 2024Article
- A Review on Acupuncture Efficiency in Human Polycystic Ovary/Ovarian Syndrome.Journal of pharmacopuncture · 2023Review
- Effects of TCM on polycystic ovary syndrome and its cellular endocrine mechanism.Frontiers in endocrinology · 2023Review
- Three-dimensional visualization of electroacupuncture-induced activation of brown adipose tissue via sympathetic innervation in PCOS rats.Chinese medicine · 2022Article
- Underlying mechanisms of acupuncture therapy on polycystic ovary syndrome: Evidences from animal and clinical studies.Frontiers in endocrinology · 2022Review
- Effect of Acupuncture on Polycystic Ovary Syndrome in Animal Models: A Systematic Review.Evidence-based complementary and alternative medicine : eCAM · 2021Review
- Ovary Development: Insights From a Three-Dimensional Imaging Revolution.Frontiers in cell and developmental biology · 2021Review
- Effect of Electroacupuncture on Reproductive Disorders and Insulin Resistance in a Murine Polycystic Ovary Syndrome Model.Evidence-based complementary and alternative medicine : eCAM · 2021Article
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Authors and funding
13 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Low-frequency electro-acupuncture (EA) has been shown to restore ovulation in patients with polycystic ovary syndrome (PCOS), and previous animal experiments showed that EA improves ovarian blood flow and angiogenesis. We performed EA for 4 weeks in dihydrotestosterone (DHT)-induced PCOS-like rats and investigated the three-dimensional (3D) ovarian innervation to determine the role of innervation in folliculogenesis and vascularity. Ovarian tissues were made transparent following the CUBIC 3D tissue-clearing protocol and were immunostained using antibodies against platelet endothelial cell adhesion molecule-1 and tyrosine hydroxylase to visualize the ovarian vasculature and innervation, respectively. This was followed by 3D imaging using lightsheet microscopy and analysis using the Imaris software. In control rats, ovarian innervation increased with age, and the neuronal branching started from the ovarian hilum and reached the individual follicles at different follicle stages. At the individual follicle level, each follicle was mainly innervated by one neuronal fiber. Compared with control rats, ovaries from DHT-treated PCOS-like rats had more antral follicles and fewer preovulatory follicles and corpora lutea. Furthermore, PCOS ovaries showed decreased innervation of blood vessels near the hilum and the surrounding individual antral follicles. EA in PCOS-like rats led to increased numbers of preovulatory follicles and corpora lutea together with increased innervation of blood vessels near the hilum. To determine the role of ovarian innervation, we further performed unilateral sectioning of the superior ovarian nerve (SON) in PCOS + EA rats and found that the left sectioned ovary had fewer preovulatory follicles and corpora lutea compared with those in the right non-sectioned ovary. In conclusion, ovarian innervation likely played an important role in folliculogenesis, and EA might restore PCOS pathophysiology by regulating ovarian innervation, at least partially mediated through the SON.
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