ArticleParasites & vectors2020
Detection of DNA of filariae closely related to Mansonella perstans in faecal samples from wild non-human primates from Cameroon and Gabon.
Article in Parasites & vectors, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 19 citations in OpenAlex.
- Polymorphic marker regions support divergence of Mansonella sp. "DEUX" and M. perstans.Parasites & vectors · 2026Article
- Development and Assessment of a Multiple-Analysis System for Diagnosing Malaria and Other Blood Parasite Infections in Humans and Non-Human Primates.Diagnostics (Basel, Switzerland) · 2025Article
- Development and validation of a long-read metabarcoding platform for the detection of filarial worm pathogens of animals and humans.BMC microbiology · 2024Article
- Unveiling zoonotic threats: molecular identification ofFrontiers in veterinary science · 2024Article
- Whole genome analysis of two sympatric humanFrontiers in cellular and infection microbiology · 2023Article
- Molecular Detection and Characterization of Intestinal and Blood Parasites in Wild Chimpanzees (Animals : an open access journal from MDPI · 2021Article
- Soil-transmitted helminth infections in free-ranging non-human primates from Cameroon and Gabon.Parasites & vectors · 2021Article
- Limitations of PCR detection of filarial DNA in human stools from subjects non-infected with soil-transmitted helminths.Parasite (Paris, France) · 2021Article
- New Molecular Data on Filaria and itsPathogens (Basel, Switzerland) · 2020Article
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Authors and funding
15 authors at 4 institutions in 3 countries.
Funding
Abstract
backgroundThe Onchocercidae is a family of filarial nematodes with several species of medical or veterinary importance. Microfilariae are found in the blood and/or the dermis and are usually diagnosed in humans by microscopy examination of a blood sample or skin biopsy. The main objectives of this study were to evaluate whether filariae DNA can be detected in faecal samples of wild non-human primates (NHPs), whether the detected parasites were closely related to those infecting humans and whether filarial DNA detection in faeces is associated with co-infections with nematodes (Oesophagostumum sp. and Necator sp.) known to cause blood loss while feeding on the host intestinal mucosa.
methodsA total of 315 faecal samples from 6 species of NHPs from Cameroon and Gabon were analysed. PCRs targeted DNA fragments of cox1 and 12S rDNA genes, to detect the presence of filariae, and the internal transcribed spacer 2 (ITS2), to detect the presence of Oesophagostomum sp. and Necator sp. infections.
resultsAmong the 315 samples analysed, 121 produced sequences with > 90% homology with Onchocercidae reference sequences. However, 63% of the 12S rDNA and 78% of the cox1 gene sequences were exploitable for phylogenetic analyses and the amplification of the 12S rDNA gene showed less discriminating power than the amplification of the cox1 fragment. Phylogenetic analyses showed that the cox1 sequences obtained from five chimpanzee DNA faecal samples from Gabon and two from Cameroon cluster together with Mansonella perstans with high bootstrap support. Most of the remaining sequences clustered together within the genus Mansonella, but the species could not be resolved. Among the NHP species investigated, a significant association between filarial DNA detection and Oesophagostomum sp. and Necator sp. infection was observed only in gorillas.
conclusionsTo our knowledge, this is the first study reporting DNA from Mansonella spp. in faecal samples. Our results raise questions about the diversity and abundance of these parasites in wildlife, their role as sylvatic reservoirs and their potential for zoonotic transmission. Future studies should focus on detecting variants circulating in both human and NHPs, and improve the molecular information to resolve or support taxonomy classification based on morphological descriptions.
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