Evidence map›Paper›PMID 32545686›Full record

ArticleSensors (Basel, Switzerland)2020

Multiple Bacteria Identification in the Point-of-Care: an Old Method Serving a New Approach.

Sara Viveiros, Mónica Rodrigues, Débora Albuquerque, Sofia A M Martins, Susana Cardoso, Verónica C Martins

Abstract read
In one paragraph

Article in Sensors (Basel, Switzerland), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. FTA-LAMP based biosensor for a rapid in-field detection ofFrontiers in bioengineering and biotechnology · 2024
    Article
  5. Article
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Sara ViveirosInstituto Superior Técnico, University of Lisbon, 1049-001 Lisboa, Portugal.ORCID 0000-0001-9636-2812
Mónica RodriguesCentre for Ecology, Evolution and Environmental Changes, Faculdade de Ciências, Universidade de Lisboa, 1749-016 Lisboa, Portugal.
Débora AlbuquerqueInstituto Superior Técnico, University of Lisbon, 1049-001 Lisboa, Portugal.
Sofia A M MartinsINESC-MN- Microsystems and Nanotechnologies, 1000-029 Lisboa, Portugal.ORCID 0000-0003-0237-6370
Susana CardosoInstituto Superior Técnico, University of Lisbon, 1049-001 Lisboa, Portugal.ORCID 0000-0001-6913-6529
Verónica C MartinsINESC-MN- Microsystems and Nanotechnologies, 1000-029 Lisboa, Portugal.ORCID 0000-0002-7032-599X

Funding

Fundação para a Ciência e a Tecnologia PD/BD/143024/2018Fundação para a Ciência e a Tecnologia PO NORTE-01-0145-FEDER-22090
6 · The paper itself

Abstract

The accurate diagnosis of bacterial infections is of critical importance for effective treatment decisions. Due to the multietiologic nature of most infectious diseases, multiplex assays are essential for diagnostics. However, multiplexability in nucleic acid amplification-based methods commonly resorts to multiple primers and/or multiple reaction chambers, which increases analysis cost and complexity. Herein, a polymerase chain reaction (PCR) offer method based on a universal pair of primers and an array of specific oligonucleotide probes was developed through the analysis of the bacterial 16S ribosomal RNA gene. The detection system consisted of DNA hybridization over an array of magnetoresistive sensors in a microfabricated biochip coupled to an electronic reader. Immobilized probes interrogated single-stranded biotinylated amplicons and were obtained using asymmetric PCR. Moreover, they were magnetically labelled with streptavidin-coated superparamagnetic nanoparticles. The benchmarking of the system was demonstrated to detect five major bovine mastitis-causing pathogens:

Indexed as

Point-of-Care SystemsAnimalsBacteriaCattleDNA, BacterialFemaleMastitis, BovineOligonucleotide ProbesPolymerase Chain ReactionRNA, Ribosomal, 16SSensitivity and SpecificityDNA, BacterialOligonucleotide ProbesRNA, Ribosomal, 16Sasymmetric PCRbacterial pathogensbiosensormagnetoresistancespin-valve

Identifiers

PMID32545686
PMCPMC7349726

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.