Evidence map›Paper›PMID 32545649›Full record

ArticleDiagnostics (Basel, Switzerland)2020

Development of a Nucleic Acid Lateral Flow Immunoassay for the Detection of Human Polyomavirus BK.

Yi-Huei Huang, Kuan-Yi Yu, Shou-Ping Huang, Hui-Wen Chuang, Wen-Zhi Lin, Juin-Hong Cherng, Yao-Wen Hung, Ming-Kung Yeh, Po-Da Hong, Cheng-Che Liu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Diagnostics (Basel, Switzerland), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.7field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 11 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Yi-Huei HuangBiomedical Engineering Program, Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei 10607, Taiwan.
Kuan-Yi YuGraduate Institute of Life Sciences, National Defense Medical Center, Taipei 11490, Taiwan.
Shou-Ping HuangDepartment of Physiology and Biophysics, Graduate Institute of Physiology, National Defense Medical Center, Taipei 11490, Taiwan.
Hui-Wen ChuangGraduate Institute of Life Sciences, National Defense Medical Center, Taipei 11490, Taiwan.
Wen-Zhi LinInstitute of Preventive Medicine, National Defense Medical Center, Taipei 11490, Taiwan.
Juin-Hong CherngDepartment and Graduate Institute of Biology and Anatomy, National Defense Medical Center, Taipei 11490, Taiwan.
Yao-Wen HungInstitute of Preventive Medicine, National Defense Medical Center, Taipei 11490, Taiwan.
Ming-Kung YehGraduate Institute of Life Sciences, National Defense Medical Center, Taipei 11490, Taiwan.
Po-Da HongBiomedical Engineering Program, Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei 10607, Taiwan.
Cheng-Che LiuDepartment of Physiology and Biophysics, Graduate Institute of Physiology, National Defense Medical Center, Taipei 11490, Taiwan.
National Defense Medical Center · TWNational Taiwan University of Science and Technology · TWNational Taipei University of Nursing and Health Science · TW

Funding

Ministry of National Defense Medical Affairs Bureau of Taiwan, Republic of China MAB-109-034Ministry of Science and Technology of Taiwan, Republic of China MOST 106-2314-B-016-014
6 · The paper itself

Abstract

The BK virus (BKV) is an emerging pathogen in immunocompromised individuals and widespread in the human population. Polymerase chain reaction is a simple and highly sensitive method for detecting BKV, but it is time consuming and requires expensive instruments and expert judgment. The lateral flow assay, a rapid, low-cost, minimal-labor, and easy-to-use diagnostic method, was successfully applied for pathogen detection. In this study, we used oligonucleotide probes to develop a simple and rapid sandwich-type lateral flow immunoassay for detecting BKV DNA within 45 minutes. The detection limit for the synthetic single-stranded DNA was 5 nM. The specificity study showed no cross-reactivity with other polyomaviruses, such as JC virus and simian virus 40. For the

Indexed as

BK polyomaviruslateral flow immunoassaynucleic acid testingpoint-of-care testing

Identifiers

PMID32545649
PMCPMC7345645
OpenAlexW3035602920

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.