ArticlePloS one2020
Lipidomics of human adipose tissue reveals diversity between body areas.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 23 citations in OpenAlex.
- Fatty acid synthesis restricts HIV-1 infection through regulation of the nuclear envelope in CD4+ T cells.bioRxiv : the preprint server for biology · 2026Article
- Adiposome Lipidomic Shifts Following Bariatric Surgery in Association With Weight Loss and Cardiometabolic Benefit.The Journal of clinical endocrinology and metabolism · 2026Article
- Exploring Adipose Tissue Complexity Through Omics Approaches: Implications for Health and Disease.Cells · 2026Review
- Lipedema as a disorder of adipose tissue heterogeneity: insights from single-cell and spatial transcriptomics.Frontiers in cell and developmental biology · 2026Review
- Lipid Profile Characterization of Human Micro-Fragmented Adipose Tissue via Untargeted Lipidomics.Biomolecules · 2025Article
- Testing of rapid evaporative mass spectrometry for histological tissue classification and molecular diagnostics in a multi-site study.British journal of cancer · 2024Article
- White adipose tissue remodeling in Little Brown Myotis (Myotis lucifugus) with white-nose syndrome.Metabolomics : Official journal of the Metabolomic Society · 2024Article
- White adipocyte dysfunction and obesity-associated pathologies in humans.Nature reviews. Molecular cell biology · 2024Review
- Drug screen identifies verteporfin as a regulator of lipid metabolism in macrophage foam cells.Scientific reports · 2023Article
- Lipid remodeling of adipose tissue in metabolic health and disease.Experimental & molecular medicine · 2023Review
- Effects of Different Storage Conditions on Lipid Stability in Mice Tissue Homogenates.Metabolites · 2023Article
- Precision diagnostic approach to predict 5-year risk for microvascular complications in type 1 diabetes.EBioMedicine · 2022Article
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- The Insulin-Sensitizer Pioglitazone Remodels Adipose Tissue Phospholipids in Humans.Frontiers in physiology · 2021Article
- Nonalcoholic fatty liver disease stratification by liver lipidomics.Journal of lipid research · 2021Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
aimsAdipose tissue plays a pivotal role in storing excess fat and its composition reflects the history of person's lifestyle and metabolic health. Broad profiling of lipids with mass spectrometry has potential for uncovering new knowledge on the pathology of obesity, metabolic syndrome, diabetes and other related conditions. Here, we developed a lipidomic method for analyzing human subcutaneous adipose biopsies. We applied the method to four body areas to understand the differences in lipid composition between these areas. MATERIALS AND
methodsAdipose tissue biopsies from 10 participants were analyzed using ultra-high-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry. The sample preparation optimization included the optimization of the lipid extraction, the sample amount and the sample dilution factor to detect lipids in an appropriate concentration range. Lipidomic analyses were performed for adipose tissue collected from the abdomen, breast, thigh and lower back. Differences in lipid levels between tissues were visualized with heatmaps.
resultsLipidomic analysis on human adipose biopsies lead to the identification of 186lipids in 2 mg of sample. Technical variation of the lipid-class specific internal standards were below 5%, thus indicating acceptable repeatability. Triacylglycerols were highly represented in the adipose tissue samples, and lipids from 13 lipid classes were identified. Long polyunsaturated triacylglycerols in higher levels in thigh (q<0.05), when compared with the abdomen, breast and lower back, indicating that the lipidome was area-specific.
conclusionThe method presented here is suitable for the analysis of lipid profiles in 2 mg of adipose tissue. The amount of fat across the body is important for health but we argue that also the distribution and the particular profile of the lipidome may be relevant for metabolic outcomes. We suggest that the method presented in this paper could be useful for detecting such aberrations.
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