ArticleInvestigative ophthalmology & visual science2020
Wounding Induces Facultative Opn5-Dependent Circadian Photoreception in the Murine Cornea.
Article in Investigative ophthalmology & visual science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 23 citations in OpenAlex.
- Effect of Light Wavelength on Corneal Sensitivity.Ophthalmology science · 2026Article
- Non-visual opsins and their role in circadian photoentrainment.Eye and vision (London, England) · 2026Review
- Hypoxia-induced OPN5 expression is associated with matrix-preserving responses in human scleral fibroblasts.Frontiers in neuroscience · 2026Article
- The physiological functions of OPN5m.Eye and vision (London, England) · 2025Review
- Article
- Circadian Rhythms in Murine Ocular Tissues Including Sclera Are Affected by Neurobasal A Medium Preincubation and Mouse Strain, but Not Sex.Investigative ophthalmology & visual science · 2025Article
- Direct Stimulation of Gastric Smooth Muscle Cells via GNeurogastroenterology and motility · 2025Article
- Neuropsin, TRPV4 and intracellular calcium mediate intrinsic photosensitivity in corneal epithelial cells.The ocular surface · 2025Article
- Violet Light Is Abundant Outdoors but Deficient Indoors in Modern Lifestyle in Tokyo.International journal of environmental research and public health · 2025Article
- Light Sensing beyond Vision: Focusing on a Possible Role for the FICZ/AhR Complex in Skin Optotransduction.Cells · 2024Review
- Introduction and reflections on the comparative physiology of sleep and circadian rhythms.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2024Article
- Ocular and extraocular roles of neuropsin in vertebrates.Trends in neurosciences · 2022Review
- Circadian Oscillations in the Murine Preoptic Area Are Reset by Temperature, but Not Light.Frontiers in physiology · 2022Article
- Melatonin Adjusts the Phase of Mouse Circadian Clocks in the Cornea Both Ex Vivo and In Vivo.Journal of biological rhythms · 2021Article
- Evolutionary Constraint on Visual and Nonvisual Mammalian Opsins.Journal of biological rhythms · 2021Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Purpose: Autonomous molecular circadian clocks are present in the majority of mammalian tissues. These clocks are synchronized to phases appropriate for their physiologic role by internal systemic cues, external environmental cues, or both. The circadian clocks of the in vivo mouse cornea synchronize to the phase of the brain's master clock primarily through systemic cues, but ex vivo corneal clocks entrain to environmental light cycles. We evaluated the underlying mechanisms of this difference. Methods: Molecular circadian clocks of mouse corneas were evaluated in vivo and ex vivo for response to environmental light. The presence of opsins and effect of genetic deletion of opsins were evaluated for influence on circadian photoresponses. Opn5-expressing cells were identified using Opn5Cre;Ai14 mice and RT-PCR, and they were characterized using immunocytochemistry. Results: Molecular circadian clocks of the cornea remain in phase with behavioral circadian locomotor rhythms in vivo but are photoentrainable in tissue culture. After full-thickness incision or epithelial debridement, expression of the opsin photopigment Opn5 is induced in the cornea in a subset of preexisting epithelial cells adjacent to the wound site. This induction coincides with conferral of direct, short-wavelength light sensitivity to the circadian clocks throughout the cornea. Conclusions: Corneal circadian rhythms become photosensitive after wounding. Opn5 gene function (but not Opn3 or Opn4 function) is necessary for induced photosensitivity. These results demonstrate that opsin-dependent direct light sensitivity can be facultatively induced in the murine cornea.
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