ArticlePLoS neglected tropical diseases2020
High-throughput multiplex qPCRs for the surveillance of zoonotic species of canine hookworms.
Article in PLoS neglected tropical diseases, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 1 synthesis or guideline pooled it, 57 citations in OpenAlex.
- A systematic review and meta-analysis of human and zoonotic dog soil-transmitted helminth infections in Australian Indigenous communities.PLoS neglected tropical diseases · 2022Pooled it
- Environmental contamination with canine soil-transmitted helminths in New Zealand urban areas: A nationwide molecular, spatial, and risk factor analysis survey.One health (Amsterdam, Netherlands) · 2026Article
- Imported zoonotic Ancylostoma ceylanicum and Ancylostoma braziliense infections in a cat in Romania.Parasites & vectors · 2026Article
- Efficacy evaluation of a new oral chewable tablet containing fluralaner, moxidectin, and pyrantel (BRAVECTOParasites & vectors · 2026Article
- Surveillance of soil-transmitted helminths and other intestinal parasites in shelter dogs, Mississippi, USA.One health (Amsterdam, Netherlands) · 2025Article
- Efficacy of Simparica Trio® against induced infections of Ancylostoma braziliense and Ancylostoma ceylanicum in dogs.Parasites & vectors · 2025Article
- Prevalence of gastrointestinal nematode parasites of dogs and associated risk factors in Gondar town, Northwest Ethiopia.Heliyon · 2025Article
- Identification of Ancylostoma caninum in Domestic Dogs from Ecuador via Various Techniques.Medical science monitor : international medical journal of experimental and clinical research · 2025Article
- The occurrence of cross-host species soil-transmitted helminth infections in humans and domestic/livestock animals: A systematic review.PLOS global public health · 2025Article
- Single and co-infections by Anaplasma platys, Babesia vogeli, Ehrlichia canis, and Hepatozoon canis in dogs from Rio Grande do Norte, Brazil: frequency and molecular characterization.Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria · 2025Article
- Prevalence and risk factors associated with zoonotic gastrointestinal helminths transmitted by cats in Jabodetabek, Indonesia.Open veterinary journal · 2024Article
- Anthroponotic and Zoonotic Hookworm DNA in an Indigenous Community in Coastal Ecuador: Potential Cross-Transmission between Dogs and Humans.Pathogens (Basel, Switzerland) · 2024Article
- Zoonotic Ancylostoma ceylanicum Infection in Coyotes from Guanacaste Conservation Area, Costa Rica, 2021.Emerging infectious diseases · 2024Article
- Benzimidazole F167Y polymorphism in the canine hookworm, Ancylostoma caninum: Widespread geographic, seasonal, age, and breed distribution in United States and Canada dogs.International journal for parasitology. Drugs and drug resistance · 2024Article
- Ecoepidemiology of Ancylostoma spp. in Urban-Marginal and Rural Sectors of the Ecuadorian Coast and Prevalence of Cutaneous Larvae Migrans.Medical science monitor : international medical journal of experimental and clinical research · 2024Article
- Geographic Distribution of Human Infections with Zoonotic Ancylostoma ceylanicum and Anthropophilic Hookworms in Ecuador: A Retrospective Analysis of Archived Stool Samples.The American journal of tropical medicine and hygiene · 2024Article
- Risk Factors Associated with Soil-Transmitted Helminths in Dog Feces That Contaminate Public Areas of Warsaw, Poland.Animals : an open access journal from MDPI · 2024Article
- Modern technologies and solutions to enhance surveillance and response systems for emerging zoonotic diseases.Science in One Health · 2024Review
- A comparative field efficacy trial of three treatment programs against endo- and ectoparasites in naturally infected dogs.Frontiers in veterinary science · 2024Article
- Anthelmintic resistance in soil-transmitted helminths: One-Health considerations.Parasitology research · 2023Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The canine hookworms Ancylostoma braziliense, Ancylostoma ceylanicum, Ancylostoma caninum and Uncinaria stenocephala are not only capable of producing morbidity and mortality in dogs but are also neglected tropical zoonoses. Each hookworm species differs considerably in its geographical distribution, life cycle, biology, pathogenic impacts on both canine and human hosts, zoonotic potential, and response to treatment with anthelminthics. Here we describe the development and validation of two Taq-Man based multiplex PCR assays capable of detecting and differentiating all four canine hookworm species in faeces of naturally infected dogs. The analytical sensitivity of both assays was assessed using 10-fold serial dilutions of synthetic gene block fragments containing individual sequence targets of each hookworm species. The sensitivity of the assays and ability to detect mixed species infections were compared to a conventional PCR-Restriction Fragment Length Polymorphism based-approach when applied to laboratory and field samples from endemic areas. The qPCRs detected at least one species of hookworms in 82.4% of PCR-RFLP-negative but microscopy-positive samples. The qPCRs detected an additional 68% mixed infections with different species of canine hookworms, and additional single species infection with A. caninum (47%), U. stenocephala (33%) and A. ceylanicum (0.02%) that were missed by PCR-RFLP. These multiplex qPCR assays will assist field based epidemiological surveillance studies towards an accurate and sensitive monitoring of canine hookworm infections in dogs, to inform their species-specific zoonotic risks to populations living in endemic areas, globally.
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Registered trials
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