Evidence map›Paper›PMID 32542012›Full record

ArticlePloS one2020

Comparing serum protein levels can aid in differentiating HPV-negative and -positive oropharyngeal squamous cell carcinoma patients.

Amy Dickinson, Mayank Saraswat, Stina Syrjänen, Tiialotta Tohmola, Robert Silén, Reija Randén-Brady, Timo Carpén, Jaana Hagström, Caj Haglund, Petri Mattila and 3 more

Open access · goldAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed, 3 pooled it
1.0field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 3 syntheses or guidelines pooled it, 15 citations in OpenAlex.

  1. Pooled it
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 2 countries.

Amy DickinsonDepartment of Otorhinolaryngology-Head and Neck Surgery, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.ORCID 0000-0003-4559-7819
Mayank SaraswatTransplantation Laboratory, Haartman Institute, University of Helsinki, Helsinki, Finland.
Stina SyrjänenDepartment of Oral Pathology and Oral Radiology, University of Turku, Turku, Finland.
Tiialotta TohmolaTransplantation Laboratory, Haartman Institute, University of Helsinki, Helsinki, Finland.
Robert SilénTransplantation Laboratory, Haartman Institute, University of Helsinki, Helsinki, Finland.
Reija Randén-BradyDepartment of Pathology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
Timo CarpénDepartment of Otorhinolaryngology-Head and Neck Surgery, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
Jaana HagströmDepartment of Oral Pathology and Oral Radiology, University of Turku, Turku, Finland.
Caj HaglundDepartment of Surgery, University of Helsinki and Helsinki, University Hospital Helsinki, Helsinki, Finland.
Petri MattilaDepartment of Otorhinolaryngology-Head and Neck Surgery, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
Antti MäkitieDepartment of Otorhinolaryngology-Head and Neck Surgery, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
Sakari JoenvääräTransplantation Laboratory, Haartman Institute, University of Helsinki, Helsinki, Finland.
Suvi SilénDepartment of Otorhinolaryngology-Head and Neck Surgery, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
University of Helsinki · FIKarolinska University Hospital · SEUniversity of Turku · FI

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe surrogate immunohistochemical marker, p16INK4a, is used in clinical practice to determine the high-risk human papillomavirus (HPV) status of oropharyngeal squamous cell carcinomas (OPSCC). With a specificity of 83%, this will misclassify some patients compared with direct HPV testing. Patients who are p16INK4a-positive but HPV DNA-negative, or RNA-negative, may be unsuitable for treatment de-escalation aimed at reducing treatment-related side effects. We aimed to identify cost-effective serum markers to improve decision making for patients at risk of misclassification by p16INK4a alone.

methodsSerum proteins from pre-treatment samples of 36 patients with OPSCC were identified and quantified using label-free mass spectrometry-based proteomics. HPV-status was determined using p16INK4a/HPV DNA and E6/E7 mRNA. Serum protein expressions were compared between groups of patients according to HPV status, using the unpaired t-test with a Benjamini-Hochberg correction. ROC curves (AUC) were calculated with SPSS (v25).

resultsOf 174 serum proteins identified, complement component C7 (C7), apolipoprotein F (ApoF) and galectin-3-Binding Protein (LGALS3BP) significantly differed between HPV-positive and -negative tumors (AUC ranging from 0.84-0.87). ApoF levels were more than twice as high in the E6/E7 mRNA HPV-positive group than HPV-negative.

conclusionsSerum C7, ApoF and LGALS3BP levels discriminate between HPV-positive and HPV-negative OPSCC. Further studies are needed to validate these host immunity-related proteins as markers for HPV-associated OPSCC.

Indexed as

AgedAged, 80 and overAntigens, NeoplasmApolipoproteinsBiomarkersBiomarkers, TumorComplement C7Cyclin-Dependent Kinase Inhibitor p16Extracellular Matrix ProteinsFemaleHumansMaleMiddle AgedOropharyngeal NeoplasmsPapillomaviridaePapillomavirus InfectionsAntigens, Neoplasmapolipoprotein FApolipoproteinsBiomarkersBiomarkers, TumorComplement C7Cyclin-Dependent Kinase Inhibitor p16Extracellular Matrix ProteinsLGALS3BP protein, human

Identifiers

PMID32542012
PMCPMC7295232
OpenAlexW3034779282

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.