ArticleInternational journal of obesity (2005)2020
Quantifying the human diet in the crosstalk between nutrition and health by multi-targeted metabolomics of food and microbiota-derived metabolites.
Article in International journal of obesity (2005), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 52 citations in OpenAlex.
- A Polyphenol-Rich Diet Increases the Gut Microbiota Metabolite Indole 3-Propionic Acid in Older Adults with Preserved Kidney Function.Molecular nutrition & food research · 2022Trial
- Characterization of a metabolomics signature of the brain-health-promoting MIND diet in older persons.The journal of prevention of Alzheimer's disease · 2026Article
- A Validated LC-MS/MS Method for Quantifying Phenolic Acids, Lignans, and Enterolignans from Human Fecal Samples.ACS nutrition science · 2026Article
- Sources of variation in the serum metabolome of female participants of the HUNT2 study.Communications biology · 2024Article
- Wine, Polyphenols, and the Matrix Effect: Is Alcohol Always the Same?International journal of molecular sciences · 2024Review
- Article
- Effect of the novel anti-NGF monoclonal antibody DS002 on the metabolomics of pain mediators, cartilage and bone.Frontiers in pharmacology · 2024Article
- Causal relationship between plasma metabolites and carpal tunnel syndrome risk: evidence from a mendelian randomization study.Frontiers in molecular biosciences · 2024Article
- Triangulating nutrigenomics, metabolomics and microbiomics toward personalized nutrition and healthy living.Human genomics · 2023Review
- Exploring Human Metabolome after Wine Intake-A Review.Molecules (Basel, Switzerland) · 2023Review
- Moderate Wine Consumption and Health: A Narrative Review.Nutrients · 2022Review
- Can individual fatty acids be used as functional biomarkers of dairy fat consumption in relation to cardiometabolic health? A narrative review.The British journal of nutrition · 2022Review
- Plasma metabolite profiles related to plant-based diets and the risk of type 2 diabetes.Diabetologia · 2022Article
- High-Throughput Method for Wide-Coverage and Quantitative Phenolic Fingerprinting in Plant-Origin Foods and Urine Samples.Journal of agricultural and food chemistry · 2022Article
- Metabolomics application for the design of an optimal diet.Journal of preventive medicine and hygiene · 2022Review
- Metabolomics: A Powerful Tool to Understand the Schizophrenia Biology.Advances in experimental medicine and biology · 2022Article
- Assessing Adherence to Healthy Dietary Habits Through the Urinary Food Metabolome: Results From a European Two-Center Study.Frontiers in nutrition · 2022Article
- Dietary Management of Heart Failure: DASH Diet and Precision Nutrition Perspectives.Nutrients · 2021Review
- Food and Microbiota Metabolites Associate with Cognitive Decline in Older Subjects: A 12-Year Prospective Study.Molecular nutrition & food research · 2021Article
- Metabolomics Technologies for the Identification and Quantification of Dietary Phenolic Compound Metabolites: An Overview.Antioxidants (Basel, Switzerland) · 2021Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMetabolomics is a powerful tool for investigating the association between nutrition and health status. Although urine is commonly employed for studying the metabolism and transformation of food components, the use of blood samples could be preferable to gain new insights into the bioavailability of diet-derived compounds and their involvement in health. However, the chemical complexity of blood samples hinders the analysis of this biological fluid considerably, which makes the development of novel and comprehensive analytical methods mandatory.
methodsIn this work, we optimized a multi-targeted metabolomics platform for the quantitative and simultaneous analysis of 450 food-derived metabolites by ultra-high performance liquid chromatography coupled to tandem mass spectrometry. To handle the chemical complexity of blood samples, three complementary extraction methods were assayed and compared in terms of recovery, sensitivity, precision and matrix effects with the aim of maximizing metabolomics coverage: protein precipitation, reversed solid-phase extraction, and hybrid protein precipitation with solid-phase extraction-mediated phospholipid removal.
resultsAfter careful optimization of the extraction conditions, protein precipitation enabled the most efficient and high-throughput extraction of the food metabolome in plasma, although solid-phase extraction-based protocols provided complementary performance for the analysis of specific polyphenol classes. The developed method yielded accurate recovery rates with negligible matrix effects, and good linearity, as well as high sensitivity and precision for most of the analyzed metabolites.
conclusionsThe multi-targeted metabolomics platform optimized in this work enables the simultaneous detection and quantitation of 450 dietary metabolites in short-run times using small volumes of biological sample, which facilitates its application to epidemiological studies.
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