ArticleNature methods2020
Highly specific multiplexed RNA imaging in tissues with split-FISH.
Article in Nature methods, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 57 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
57 citing papers in PubMed, 123 citations in OpenAlex.
- Immunophenotyping by Spatial Biology.Methods in molecular biology (Clifton, N.J.) · 2027Article
- Sequencing-free spatial profiling of post-transcriptional regulation in fresh tissues using nanoneedle arrays.Nature biomedical engineering · 2026Article
- Spatial multi-omics analyses of the tumor immune microenvironment: advances and novel therapeutic avenues.Molecular cancer · 2026Review
- In situ imaging of proximal biomolecules via Proximity Anchored Modules Assembly.Journal of nanobiotechnology · 2026Article
- The applications of single-cell and spatial transcriptomics in neuroscience and brain disorders.Neuroscience and biobehavioral reviews · 2026Review
- Spatial architecture of autism pathogenesis reveals mosaic structural disarray during early development.Nature communications · 2026Article
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- A Guide for Spatial Omics Technologies: Innovation, Evaluation, and Application.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Spatial perturb-seq: single-cell functional genomics within intact tissue architecture.Nature communications · 2026Article
- Tetrahedral DNA dendritic nanostructure-enhanced FISH for high-speed, sensitive spatial transcriptomics.Nature communications · 2025Article
- Live-cell omics with Raman spectroscopy.Microscopy (Oxford, England) · 2025Review
- One probe fits all: a highly customizable modular RNA in situ hybridization platform expanding the application of SABER DNA probes.Development (Cambridge, England) · 2025Article
- Homebuilt Imaging-Based Spatial Transcriptomics: Tertiary Lymphoid Structures as a Case Example.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Advancements in pathology: Digital transformation, precision medicine, and beyond.Journal of pathology informatics · 2025Review
- Large field of view and spatial region of interest transcriptomics in fixed tissue.Communications biology · 2024Article
- Spatial Dissection of the Immune Landscape of Solid Tumors to Advance Precision Medicine.Cancer immunology research · 2024Review
- Accurate single-molecule spot detection for image-based spatial transcriptomics with weakly supervised deep learning.Cell systems · 2024Article
- Applications of single‑cell omics and spatial transcriptomics technologies in gastric cancer (Review).Oncology letters · 2024Review
- Highly sensitive spatial transcriptomics using FISHnCHIPs of multiple co-expressed genes.Nature communications · 2024Article
- Spatial Omics: Navigating Neuroscience Research into the New Era.Advances in neurobiology · 2024Review
Corrections and comments
- Erratum issued
- Erratum issued
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We present split-FISH, a multiplexed fluorescence in situ hybridization method that leverages a split-probe design to achieve enhanced specificity. Split-FISH reduces off-target background fluorescence, decreases false positives and enables accurate RNA profiling in uncleared tissues. We demonstrate the efficacy of split-FISH on various mouse tissues by quantifying the distribution and abundance of 317 genes in single cells and reveal diverse localization patterns for spatial regulation of the transcriptome in complex tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.