ArticleScientific reports2020
Isolation and propagation of leptospires at 37 °C directly from the mammalian host.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed.
- Glutamine from plasma-like medium fuels nitrogen metabolism and influences proliferation in Leptospira interrogans.PLoS pathogens · 2026Article
- Clinical and molecular characterization of an outbreak of leptospirosis in dogs from Los Angeles County, California, USA, 2021.Journal of clinical microbiology · 2026Article
- Identification and characterization of small RNAs expressed by Leptospira borgpetersenii serovar Hardjo and their conservation in the genus Leptospira.BMC microbiology · 2026Article
- TheInfection and immunity · 2026Article
- CRISPR-based mutagenesis of lipopolysaccharide biosynthesis genes in Leptospira interrogans reveals gene essentiality and confirms the role of an O-antigen polymerase.Scientific reports · 2026Article
- Diagnosis of leptospirosis in animals: challenges and perspectives.Journal of clinical microbiology · 2026Review
- DMEM and EMEM as alternate growth media for pathogenic Leptospira.PLoS neglected tropical diseases · 2026Article
- Early diagnosis and treatment of leptospirosis: Optimizing clinical outcomes.The Journal of infection · 2026Review
- Leptospira and Leptospirosis: A Review of Species Classifications, Genomes, Morphological Structures, Antimicrobial Resistances, Transmissions, and Clinical Manifestations.Current microbiology · 2026Review
- cGAS-STING dependent type I IFN reduces Leptospira interrogans renal colonization in mice.PLoS pathogens · 2026Article
- In vitro and in vivo endothelial interactions of Leptospira species are markers of virulence.PLoS neglected tropical diseases · 2026Article
- EMEM and DMEM as an Alternative Media for Leptospiral Culture, Diagnostic, and Pathogenesis Studies.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Article
- Determination of Bacterial Adherence to Mouse Tissues Using a Hematogenous Model.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Leptospira and Leptospirosis.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Laboratory Diagnosis of Leptospirosis.Methods in molecular biology (Clifton, N.J.) · 2026Review
- Preparation of HAN Medium for the Isolation of Leptospira Directly from Infected Hosts.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Complement C3 deficiency enhances renal leptospiral load and inflammation while impairing T cell differentiation during chronicInfection and immunity · 2025Article
- The Role of Hydrometeorological Factors in Leptospirosis Transmission in Central Java, Indonesia.Journal of preventive medicine and public health = Yebang Uihakhoe chi · 2025Article
- Shaping the future ofJournal of medical microbiology · 2025Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The causative agent of leptospirosis includes multiple serovars and species of pathogenic leptospires that are excreted via urine from reservoir hosts of infection. Primary isolation takes weeks to months, and is limited to semi-solid media at 28-30 °C. Here we present an alternative media formulation, HAN, compared to commercially available EMJH and the more specialized T80/40/LH media formulations, in semi-solid and liquid compositions, for the primary isolation of two diverse species and serovars of pathogenic leptospires directly from host kidney tissue. All three media types supported the isolation and propagation of L. interrogans serovar Copenhageni strain IC:20:001 in semi-solid media at 29 °C. However, only HAN and T80/40/LH supported the growth of L. borgpetersenii serovar Hardjo strain HB15B203 at 29 °C. In addition, HAN supported primary isolation at 37 °C. Both T80/40/LH and HAN supported primary isolation of strain IC:20:001 in liquid media at 29 °C but only HAN supported growth of strain HB15B203 in liquid media, at both 29 and 37 °C. HAN media supports the primary isolation of fastidious pathogenic leptospires directly from infected host tissue at either 29 or 37 °C: this formulation represents a more defined media for the continued optimization of growth factors required to support the primary isolation of the large and diverse range of species and serovars within the genus Leptospira circulating within domestic and wild animal populations.
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