Evidence map›Paper›PMID 32541787›Full record

ArticleScientific reports2020

Improvement in Detection Limit for Lateral Flow Assay of Biomacromolecules by Test-Zone Pre-enrichment.

Yi Zhang, Xiao Liu, Lingling Wang, Hanjie Yang, Xiaoxiao Zhang, Chenglong Zhu, Wenlong Wang, Lijing Yan, Bowei Li

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Aspergillus Mycotoxins: The Major Food Contaminants.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025
    Review
  6. Article
  7. Lateral Flow Immunosensing ofMicroorganisms · 2024
    Article
  8. Review
  9. Article
  10. Review
  11. Review
  12. Article
  13. Engineering Innovative Interfaces for Point-of-Care Diagnostics.Current opinion in colloid & interface science · 2023
    Review
  14. Article
  15. Review
  16. Review
  17. Article
  18. Article
  19. Article
  20. Osmotic Processor for Enabling Sensitive and Rapid Biomarker DetectionFrontiers in bioengineering and biotechnology · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Yi ZhangState Key Laboratory of Food Science and Technology, International Joint Laboratory on Food Safety, Collaborative innovation center of food safety and quality control in Jiangsu Province, Institute of Analytical Food Safety, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, PR China. zhangyijnu@jiangnan.edu.cn.
Xiao LiuState Key Laboratory of Food Science and Technology, International Joint Laboratory on Food Safety, Collaborative innovation center of food safety and quality control in Jiangsu Province, Institute of Analytical Food Safety, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, PR China.
Lingling WangState Key Laboratory of Food Science and Technology, International Joint Laboratory on Food Safety, Collaborative innovation center of food safety and quality control in Jiangsu Province, Institute of Analytical Food Safety, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, PR China.
Hanjie YangState Key Laboratory of Food Science and Technology, International Joint Laboratory on Food Safety, Collaborative innovation center of food safety and quality control in Jiangsu Province, Institute of Analytical Food Safety, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, PR China.
Xiaoxiao ZhangState Key Laboratory of Food Science and Technology, International Joint Laboratory on Food Safety, Collaborative innovation center of food safety and quality control in Jiangsu Province, Institute of Analytical Food Safety, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, PR China.
Chenglong ZhuState Key Laboratory of Food Science and Technology, International Joint Laboratory on Food Safety, Collaborative innovation center of food safety and quality control in Jiangsu Province, Institute of Analytical Food Safety, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, PR China.
Wenlong WangState Key Laboratory of Food Science and Technology, International Joint Laboratory on Food Safety, Collaborative innovation center of food safety and quality control in Jiangsu Province, Institute of Analytical Food Safety, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, PR China.
Lijing YanJiangnan University Hospital, Wuxi, 214122, PR China.
Bowei LiCAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Research Center for Coastal Environmental Engineering and Technology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, PR China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Lateral flow assay (LFA) is one of the most prevalent commercially available techniques for point-of-care tests due to its simplicity, celerity, low cost and robust operation. However, conventional colorimetric LFAs have inferior limits of detection (LODs) compared to sophisticated laboratory-based assays. Here, we report a simple strategy of test-zone pre-enrichment to improve the LOD of LFA by loading samples before the conjugate pad assembly. The developed method enables visual LODs of miR-210 mimic and human chorionic gonadotropin protein, to be improved by 10-100 fold compared with a conventional LFA setup without introducing any additional instrument and reagent except for phosphate running buffer, while no obvious difference occurred for Aflatoxin B1 (AFB1). It takes about 6-8 min to enrich every 50 μL of sample diluted with phosphate running buffer, therefore we can get visual results within 20 min. We identified a parameter by modeling the entire process, the concentration of probe-analyte conjugate at test zone when signaling unit being loaded, to be important for the improvement of visual limit of detection. In addition, the test-zone pre-enrichment did not impair the selectivity when miR-210 mimic was adopted as target. Integrated with other optimization, amplification and modification of LFAs, the developed test-zone pre-enrichment method can be applied to further improve LOD of LFAs.

Indexed as

Aflatoxin B1BuffersChorionic GonadotropinImmunoassayLimit of DetectionMacromolecular SubstancesAflatoxin B1BuffersChorionic GonadotropinMacromolecular Substances

Identifiers

PMID32541787
PMCPMC7295814

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.