ArticleJournal of colloid and interface science2020
Induced fusion and aggregation of bacterial outer membrane vesicles: Experimental and theoretical analysis.
Article in Journal of colloid and interface science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 34 citations in OpenAlex.
- Direct delivery of assay reagents to extracellular vesicles in liquid biopsies for biomarker analysis.Nature protocols · 2026Review
- The Role of Oral Pathobionts' Outer Membrane Vesicles in Cancer Pathology and Therapeutic Development.Cells · 2026Review
- Membrane Vesicle-Mediated Delivery of Antibacterial Lipopeptides byACS chemical biology · 2026Article
- Environmental cues shape extracellular vesicles biogenesis and function in Streptococcus pneumoniae.BMC microbiology · 2026Article
- Harnessing Bacterial Lipid Coatings on Gold Nanoparticles for Enhanced Cell Adhesion Applications.Small science · 2026Article
- Engineering Bacterial Extracellular Vesicles as Nanoweapons to Fight against Bacterial Infections.Research (Washington, D.C.) · 2026Review
- Bioengineering modification and application of bacterial outer membrane vesicles.International journal of medical sciences · 2026Review
- Toward a better understanding of the ecological roles of extracellular vesicles from plant-associated bacteria.Applied and environmental microbiology · 2025Review
- Modification of Silver-Loaded Biodegradable Polymer Nanoparticles with Bacterial Membrane Vesicles for Treating Intracellular Bacterial Infections.Materials (Basel, Switzerland) · 2025Article
- Outer Membrane Vesicles Derived from Oral Bacteria Act as a Dagger in Host Immunity: Insight Tips from Local Diseases to Systemic Effects.International journal of biological sciences · 2025Review
- Bacterial Extracellular Vesicles (BEVs) Derived FromInternational journal of biomaterials · 2025Article
- Consistency in bacterial extracellular vesicle production: key to their application in human health.Extracellular vesicles and circulating nucleic acids · 2025Article
- Article
- Methods for the Characterization of the Colloidal Properties of Bacterial Membrane Vesicles.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Extracellular Vesicles and Bacteriophages: New Directions in Environmental Biocolloid Research.Environmental science & technology · 2023Review
- Characterizing the Transport and Surface Affinity of Extracellular Vesicles Isolated from Yeast and Bacteria in Well-Characterized Porous Media.Environmental science & technology · 2023Article
- Comparative electrokinetic properties of extracellular vesicles produced by yeast and bacteria.Colloids and surfaces. B, Biointerfaces · 2023Article
- Role ofFrontiers in microbiology · 2023Review
- The Discovery of the Role of Outer Membrane Vesicles against Bacteria.Biomedicines · 2022Review
- ClearColi as a platform for untagged pneumococcal surface protein A production: cultivation strategy, bioreactor culture, and purification.Applied microbiology and biotechnology · 2022Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Recombinantly engineered bacterial outer membrane vesicles (OMVs) are promising vaccine delivery vehicles. The diversity of exogenous antigens delivered by OMVs can be enhanced by induced fusion of OMV populations. To date there are no reports of induced fusion of bacterial OMVs. Here we measure the pH and salt-induced aggregation and fusion of OMVs and analyze the processes against the Derjaguin-Landau-Verwey-Overbeek (DLVO) colloidal stability model. Vesicle aggregation and fusion kinetics were investigated for OMVs isolated from native E. coli (Nissle 1917) and lipopolysaccharide (LPS) modified E. coli (ClearColi) strains to evaluate the effect of lipid type on vesicle aggregation and fusion. Electrolytes and low pHs induced OMV aggregation for both native and modified LPS constructs, approaching a calculated fusion efficiency of ~25% (i.e. ~1/4 of collision events lead to fusion). However, high fusion efficiency was achieved for Nissle OMVs solely with decreased pH as opposed to a combination of low pH and increased divalent counterion concentration for ClearColi OMVs. The lipid composition of the OMVs from Nissle negatively impacted fusion in the presence of electrolytes, causing higher deviations from DLVO-predicted critical coagulation concentrations with monovalent counterions. The outcome of the work is a defined set of conditions under which investigators can induce OMVs to fuse and make various combinations of vesicle compositions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.