ArticleCurrent protocols in microbiology2020
Genetic Manipulation and Virulence Assessment of Fusobacterium nucleatum.
Article in Current protocols in microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 41 citations in OpenAlex.
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- High-Frequency Ultrasound for Detecting Periodontal Inflammation: A Preclinical Diagnostic Accuracy Study.Journal of periodontal research · 2025Article
- The CarSR two-component system directly controlsJournal of bacteriology · 2025Article
- Identification of a Fusobacterial RNA-binding protein involved in host small RNA-mediated growth inhibition.International journal of oral science · 2025Article
- Establishment and improvement of genetic manipulation tools forEngineering microbiology · 2025Review
- Development of a conditional plasmid for gene deletion in non-modelApplied and environmental microbiology · 2025Article
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- Ultrasonographic Evaluation of Vascular Response to Mechanical Compression during Induced Gingival Inflammation.Journal of dental research · 2024Article
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- A New Method for Gene Deletion to Investigate Cell Wall Biogenesis in Fusobacterium nucleatum.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Illuminating the oral microbiome and its host interactions: tools and approaches for molecular microbiology studies.FEMS microbiology reviews · 2023Review
- Development of a xylose-inducible promoter and riboswitch combination system for manipulating gene expression inApplied and environmental microbiology · 2023Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Considered a commensal, the Gram-negative anaerobe Fusobacterium nucleatum is a key member of the oral microbiome due to its wide range of interactions with many oral microbes. While the periodontal pathogenic properties of this organism have widely been examined, its connotation with extra-oral infections, including preterm birth and colorectal cancer, has now become apparent. Nonetheless, little is known about the mechanisms of pathogenicity and the associated virulence factors of F. nucleatum, most likely due to limited genetic tools and facile methodology. Here, we describe molecular techniques for the genetic manipulation of F. nucleatum, including markerless, nonpolar gene deletion, complementation, and Tn5 transposon mutagenesis. Further, we provide methodology to assess virulence potential of F. nucleatum using a mouse model of preterm birth. © 2020 Wiley Periodicals LLC. Basic Protocol 1: Generation of a galK mutant strain Basic Protocol 2: Complementation of a mutant strain Basic Protocol 3: Tn5 transposon mutagenesis of F. nucleatum Basic Protocol 4: Mouse model of preterm birth.
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Registered trials
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