Evidence map›Paper›PMID 32539073›Full record

ArticleThe Journal of experimental medicine2020

Layilin augments integrin activation to promote antitumor immunity.

Kelly M Mahuron, Joshua M Moreau, Jeff E Glasgow, Devi P Boda, Mariela L Pauli, Victoire Gouirand, Luv Panjabi, Robby Grewal, Jacob M Luber, Anubhav N Mathur and 11 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of experimental medicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.

0numbers the graph read from it
0cells of the map it votes in
38citing papers in PubMed
2.4field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

38 citing papers in PubMed, 67 citations in OpenAlex.

  1. Article
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  5. Translational Downregulation of 5' TOP mRNAs During T Cell Exhaustion.bioRxiv : the preprint server for biology · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors at 2 institutions in 1 country.

Kelly M MahuronDepartment of Surgery, University of California, San Francisco, San Francisco, CA.
Joshua M Moreau *Department of Dermatology, University of California, San Francisco, San Francisco, CA.
Jeff E GlasgowDepartment of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA.
Devi P BodaDepartment of Dermatology, University of California, San Francisco, San Francisco, CA.
Mariela L PauliDepartment of Dermatology, University of California, San Francisco, San Francisco, CA.
Victoire GouirandDepartment of Dermatology, University of California, San Francisco, San Francisco, CA.
Luv PanjabiDepartment of Dermatology, University of California, San Francisco, San Francisco, CA.
Robby GrewalDepartment of Dermatology, University of California, San Francisco, San Francisco, CA.
Jacob M LuberDepartment of Biomedical Informatics, Harvard Medical School, Boston, MA.
Anubhav N MathurDepartment of Dermatology, University of California, San Francisco, San Francisco, CA.
Renny M FeldmanT-REX Bio, Burlingame, CA.
Eric ShifrutDepartment of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA.
Pooja MehtaDepartment of Dermatology, University of California, San Francisco, San Francisco, CA.
Margaret M LoweDepartment of Dermatology, University of California, San Francisco, San Francisco, CA.
Michael D AlvaradoDepartment of Surgery, University of California, San Francisco, San Francisco, CA.
Alexander MarsonDepartment of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA.
Meromit SingerDepartment of Data Sciences, Dana-Farber Cancer Institute, Boston, MA.
Jim WellsDepartment of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA.
Ray JuppT-REX Bio, Burlingame, CA.
Adil I DaudDepartment of Medicine, University of California, San Francisco, San Francisco, CA.
Michael D RosenblumDepartment of Dermatology, University of California, San Francisco, San Francisco, CA.
University of California, San Francisco · USHarvard University · US

Funding

Research BaseP30DK063720 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI KRUMMEL, MATTHEW F · 2003 to 2019
$21.8M
Training Program in Bioinformatics and Integrative GenomicsT32HG002295 · NHGRI · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI Peter J Park · 2001 to 2026
$15.8M
MOLECULAR AND CELLULAR IMMUNOLOGYT32AI007334 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI CYSTER, JASON G · 1988 to 2023
$11.0M
RESEARCH TRAINING IN GASTROINTESTINAL SURGERYT32DK007573 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI HARRIS, HOBART WHITAKER · 1988 to 2019
$3.0M
Elucidating the Functional Role of Layilin Expression on Regulatory T cells in SkinR21AR072195 · NIAMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI ROSENBLUM, MICHAEL DAVID · 2017 to 2018
$384k
NHGRI NIH HHS T32 HG002295NIAID NIH HHS T32 AI007334NIAMS NIH HHS R21 AR072195NIDDK NIH HHS P30 DK063720NIDDK NIH HHS T32 DK007573
6 · The paper itself

Abstract

Tumor-infiltrating CD8+ T cells mediate antitumor immune responses. However, the mechanisms by which T cells remain poised to kill cancer cells despite expressing high levels of inhibitory receptors are unknown. Here, we report that layilin, a C-type lectin domain-containing membrane glycoprotein, is selectively expressed on highly activated, clonally expanded, but phenotypically exhausted CD8+ T cells in human melanoma. Lineage-specific deletion of layilin on murine CD8+ T cells reduced their accumulation in tumors and increased tumor growth in vivo. Congruently, gene editing of LAYN in human CD8+ T cells reduced direct tumor cell killing ex vivo. On a molecular level, layilin colocalized with integrin αLβ2 (LFA-1) on T cells, and cross-linking layilin promoted the activated state of this integrin. Accordingly, LAYN deletion resulted in attenuated LFA-1-dependent cellular adhesion. Collectively, our results identify layilin as part of a molecular pathway in which exhausted or "dysfunctional" CD8+ T cells enhance cellular adhesiveness to maintain their cytotoxic potential.

Indexed as

ImmunityAnimalsCarrier ProteinsCD8-Positive T-LymphocytesCell AdhesionCell ProliferationClone CellsCytokinesCytotoxicity, ImmunologicGene EditingHumansIntegrinsLectins, C-TypeLymphocyte ActivationLymphocyte Function-Associated Antigen-1Lymphocytes, Tumor-InfiltratingCarrier ProteinsCytokinesIntegrinslayilin protein, mouseLAYN protein, humanLectins, C-TypeLymphocyte Function-Associated Antigen-1Membrane GlycoproteinsTalin

Identifiers

PMID32539073
PMCPMC7478725
OpenAlexW3034758733

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-SA
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.