ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2020
A Droplet Microfluidic System to Fabricate Hybrid Capsules Enabling Stem Cell Organoid Engineering.
Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
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Who cites it
74 citing papers in PubMed.
- Cell assays in microcompartments driven by droplet microfluidics and hydrogel technologies.Journal of biochemistry · 2026Review
- "Islet-on-a-Chip": A Microfluidic System Design for Preservation of Viability and Function of Human Islets of Langerhans - A Systematic Review.Visceral medicine · 2026Article
- Integrated Microfluidic Platform for High-Throughput Generation of Intestinal Organoids in Hydrogel Droplets.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- The Synthetic Extracellular Matrix as a Maestro of the In Vitro Stem Cell Niche: Orchestrating Fate and Function.Biomedicines · 2026Review
- From organoid culture to manufacturing: technologies for reproducible and scalable organoid production.npj biomedical innovations · 2026Review
- Organ-on-a-chip platforms for disease modeling and in vitro diagnostic applications.Frontiers in bioengineering and biotechnology · 2026Review
- Single-Cell-Derived Tumor Organoid (STO) arrays on a microfluidic chip for personalized drug screening to address heterogeneity-induced drug resistance in colorectal cancer.Microsystems & nanoengineering · 2025Article
- Accelerated maturation of branched organoids confined in collagen droplets.Lab on a chip · 2025Article
- Organoid in droplet: Production of uniform pancreatic cancer organoids from single cells.Materials today. Bio · 2025Article
- An acoustofluidic embedding platform for rapid multiphase microparticle injection.Nature communications · 2025Article
- Droplet acoustofluidics: Recent progress and challenges.Biomicrofluidics · 2025Review
- Microfluidics-Based Microcarriers for Live-Cell Delivery.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Machine Learning-Enhanced Optimization for High-Throughput Precision in Cellular Droplet Bioprinting.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Biomaterial-assisted organoid technology for disease modeling and drug screening.Materials today. Bio · 2025Review
- Highly efficient construction of monkey blastoid capsules from aged somatic cells.Nature communications · 2025Article
- Organoids-on-a-chip: microfluidic technology enables culture of organoids with enhanced tissue function and potential for disease modeling.Frontiers in bioengineering and biotechnology · 2025Review
- Developments in gastrointestinal organoid cultures to recapitulate tissue environments.Frontiers in bioengineering and biotechnology · 2025Review
- Progress in the Application of Organoids-On-A-Chip in Diseases.Organogenesis · 2024Review
- Harnessing the power of artificial intelligence for human living organoid research.Bioactive materials · 2024Review
- Hydrogel local drug delivery systems for postsurgical management of tumors:Materials today. Bio · 2024Review
14 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Organoids derived from self-organizing stem cells represent a major technological breakthrough with the potential to revolutionize biomedical research. However, building high-fidelity organoids in a reproducible and high-throughput manner remains challenging. Here, a droplet microfluidic system is developed for controllable fabrication of hybrid hydrogel capsules, which allows for massive 3D culture and formation of functional and uniform islet organoids derived from human-induced pluripotent stem cells (hiPSCs). In this all-in-water microfluidic system, an array of droplets is utilized as templates for one-step fabrication of binary capsules relying on interfacial complexation of oppositely charged Na-alginate (NaA) and chitosan (CS). The produced hybrid capsules exhibit high uniformity, and are biocompatible, stable, and permeable. The established system enables capsule production, 3D culture, and self-organizing formation of human islet organoids in a continuous process by encapsulating pancreatic endocrine cells from hiPSCs. The generated islet organoids contain islet-specific α- and β-like cells with high expression of pancreatic hormone specific genes and proteins. Moreover, they exhibit sensitive glucose-stimulated insulin secretion function, demonstrating the capability of these binary capsules to engineer human organoids from hiPSCs. The proposed system is scalable, easy-to-operate, and stable, which can offer a robust platform for advancing human organoids research and translational applications.
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