ArticleJournal of industrial microbiology & biotechnology2020
Rational modification of the carbon metabolism of Corynebacterium glutamicum to enhance L-leucine production.
Article in Journal of industrial microbiology & biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 25 citations in OpenAlex.
- Multistep metabolic engineeredSynthetic and systems biotechnology · 2026Article
- Systems metabolic engineering of Corynebacterium glutamicum for efficient production of L-isoleucine.Journal of animal science and biotechnology · 2026Article
- Improved Natamycin Production inMicroorganisms · 2025Article
- Branched-chain amino acids: physico-chemical properties, industrial synthesis and role in signaling, metabolism and energy production.Amino acids · 2024Review
- HyCas9-12aGEP: an efficient genome editing platform forFrontiers in bioengineering and biotechnology · 2024Article
- Construction of a plasmid-free L-leucine overproducing Escherichia coli strain through reprogramming of the metabolic flux.Biotechnology for biofuels and bioproducts · 2023Article
- Metabolic engineering of Corynebacterium glutamicum for producing branched chain amino acids.Microbial cell factories · 2021Review
- Effect of fed-batch and chemostat cultivation processes ofBioengineered · 2021Article
- Effect of low-level ultrasound treatment on the production of L-leucine byBioengineered · 2021Article
- L-valine production in Corynebacterium glutamicum based on systematic metabolic engineering: progress and prospects.Amino acids · 2021Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
L-Leucine is an essential amino acid that has wide and expanding applications in the industry. It is currently fast-growing market demand that provides a powerful impetus to further increase its bioconversion productivity and production stability. In this study, we rationally engineered the metabolic flux from pyruvate to L-leucine synthesis in Corynebacterium glutamicum to enhance both pyruvate availability and L-leucine synthesis. First, the pyc (encoding pyruvate carboxylase) and avtA (encoding alanine-valine aminotransferase) genes were deleted to weaken the metabolic flux of the tricarboxylic acid cycle and reduce the competitive consumption of pyruvate. Next, the transcriptional level of the alaT gene (encoding alanine aminotransferase) was down regulated by inserting a terminator to balance L-leucine production and cell growth. Subsequently, the genes involved in L-leucine biosynthesis were overexpressed by replacing the native promoters P
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.