ArticleEuropean biophysics journal : EBJ2020
Influence of cholesterol on electroporation in lipid membranes of giant vesicles.
Article in European biophysics journal : EBJ, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 26 citations in OpenAlex.
- Simulation of intracellular delivery through permeabilized multivesicular vesicles.European biophysics journal : EBJ · 2026Article
- Electric field-induced rupture kinetics of giant unilamellar vesicles with varying gramicidin A content in membrane.PloS one · 2026Article
- Electroporation in lipid vesicles by varying PEG-grafted lipids in their membranes: an experimental and simulation study.RSC advances · 2025Article
- Optimizing electroporation via pulse modulation: a molecular dynamics study.European biophysics journal : EBJ · 2025Article
- Dimensions, stability, and deformability of DOPC-cholesterol giant unilamellar vesicles formed by droplet transfer.Open research Europe · 2025Article
- Probability and kinetics of rupture and electrofusion in giant unilamellar vesicles under various frequencies of direct current pulses.PloS one · 2024Article
- Liposomes Loaded with Green Tea Polyphenols-Optimization, Characterization, and Release Kinetics Under Conventional Heating and Pulsed Electric Fields.Food and bioprocess technology · 2023Article
- Passive transport of CaBiophysical journal · 2023Article
- Effects of cholesterol on the anionic magnetite nanoparticle-induced deformation and poration of giant lipid vesicles.RSC advances · 2022Article
- All-atom molecular dynamics simulations of the combined effects of different phospholipids and cholesterol content on electroporation.RSC advances · 2022Article
- Effects of sugar concentration on the electroporation, size distribution and average size of charged giant unilamellar vesicles.European biophysics journal : EBJ · 2022Article
- Effects of cholesterol on the size distribution and bending modulus of lipid vesicles.PloS one · 2022Article
- Recent developments in the kinetics of ruptures of giant vesicles under constant tension.RSC advances · 2021Review
- Analysis of purification of charged giant vesicles in a buffer using their size distribution.The European physical journal. E, Soft matter · 2021Article
- An investigation into the critical tension of electroporation in anionic lipid vesicles.European biophysics journal : EBJ · 2021Article
- Effects of osmotic pressure on the irreversible electroporation in giant lipid vesicles.PloS one · 2021Article
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Authors and funding
5 authors at 1 institution in 1 country.
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Abstract
Irreversible electroporation (IRE) is primarily a nonthermal ablative technology that uses a series of high-voltage and ultra-short pulses with high-frequency electrical energy to induce cell death. This paper presents the influence of cholesterol on the IRE-induced probability of pore formation and the rate constant of pore formation in giant unilamellar vesicles (GUVs). The GUVs are prepared by a mixture of dioleoylphosphatidylglycerol (DOPG), dioleoylphosphatidylcholine (DOPC) and cholesterol using the natural swelling method. An IRE signal of frequency 1.1 kHz is applied to the membranes of GUVs. The probability of pore formation and the rate constant of pore formation events are obtained using statistical analysis from several single GUVs. The time-dependent fraction of intact GUVs among all those examined is fitted to a single exponential decay function from where the rate constant of pore formation is calculated. The probability of pore formation and the rate constant of pore formation decreases with an increase in cholesterol content in the membranes of GUVs. Theoretical equations are fitted to the tension-dependent rate constant of pore formation and to the probability of pore formation, which allows us to obtain the line tension of membranes. The obtained line tension increases with an increase in cholesterol in the membranes. The increase in the energy barrier of the prepore state, due to the increase of cholesterol in membranes, is the main factor explaining the decrease in the rate constant of pore formation.
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