Evidence map›Paper›PMID 32535660›Full record

ArticleJournal of anesthesia2020

Functional analysis of newly identified RYR1 variants in patients susceptible to malignant hyperthermia.

Yuko Noda, Toshimichi Yasuda, Rieko Kanzaki, Hirotsugu Miyoshi, Keiko Mukaida, Satoshi Kamiya, Atsushi Morio, Hiroshi Hamada, Masashi Kawamoto, Yasuo M Tsutsumi

RetractedAbstract readRetracted Publication
PubMed Publisher
In one paragraph

Article in Journal of anesthesia, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 56% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 1 country.

Yuko NodaDepartment of Anesthesiology and Critical Care, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8551, Japan. nodananoda0724@yahoo.co.jp.ORCID 0000-0002-0624-8299
Toshimichi YasudaDepartment of Anesthesiology and Critical Care, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8551, Japan.
Rieko KanzakiDepartment of Anesthesiology and Critical Care, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8551, Japan.
Hirotsugu MiyoshiDepartment of Anesthesiology and Critical Care, Hiroshima University Hospital, Hiroshima, Japan.
Keiko MukaidaDepartment of Anesthesiology, Hiroshima Prefectural Rehabilitation Center, Higashi-hiroshima, Japan.
Satoshi KamiyaDepartment of Anesthesiology and Critical Care, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8551, Japan.
Atsushi MorioDepartment of Anesthesiology and Critical Care, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8551, Japan.
Hiroshi HamadaDepartment of Anesthesiology and Critical Care, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8551, Japan.
Masashi KawamotoMedical Corporation JR Hiroshima Hospital, Hiroshima, Japan.
Yasuo M TsutsumiDepartment of Anesthesiology and Critical Care, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8551, Japan.
Hiroshima University · JPHiroshima General Hospital · JPHiroshima Prefectural Rehabilitation Center · JPHiroshima University Hospital · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeThis study aimed to evaluate whether the three ryanodine receptor type 1 (RYR1) variants (p.Ser2345Thr, p.Ser2345Arg, and p.Lys3367Arg) which we identified in Japanese malignant hyperthermia (MH) patients with a clinical grading scale rank of 6 were causative for MH.

methodsWe prepared human embryonic kidney (HEK)-293 cells transfected with wild-type RYR1 or one of the RYR1 variants, along with myotubes cultured from muscle pieces. Calcium kinetics were examined by calculating the 340/380-nm ratio under various caffeine and 4-chloro-m-cresol (4CmC) concentrations with the ratiometric dye Fura-2 AM. Half-maximal effective concentration (EC

resultsIn functional analysis using HEK-293 cells, we found significant reductions in the EC

conclusionsp.Ser2345Thr and p.Ser2345Arg appear capable of causing a calcium metabolism disorder that leads to the onset of MH, and p.Ser2345Arg can be considered as a diagnostic mutation, because it meets the European Malignant Hyperthermia Group criteria. However, patients with p.Lys3367Arg might have mutations in genes other than RYR1 that are capable of causing MH.

Indexed as

Functional analysisMalignant hyperthermiaRyanodine receptor type 1

Identifiers

PMID32535660
OpenAlexW3035349403

What OpenQuestion holds

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Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.