ArticleJournal of molecular biology2020
Local c-di-GMP Signaling in the Control of Synthesis of the E. coli Biofilm Exopolysaccharide pEtN-Cellulose.
Article in Journal of molecular biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 81 citations in OpenAlex.
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- Bioengineering microbial cellulose synthesis pathways as biofactory for industrial-scale cellulose production: a review on current advances.World journal of microbiology & biotechnology · 2026Review
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- Dual-function enzyme acts as a global c-di-GMP sink and local anti sigma factor antagonist to drive cellular differentiation.PLoS genetics · 2026Article
- A dual-threshold system relying on multiple c-di-GMP metabolic enzymes controls cell fate of a cyanobacterium.PLoS biology · 2026Article
- Oxygen-responsive KoBdcA/KoBdcB/KoBpdA system regulates c-di-GMP levels to control bacterial cellulose biosynthesis and motility inMicrobiology spectrum · 2026Article
- High-resolution visualization of biofilm matrix development in space and time using fluorescent stains for cellulose.NPJ biofilms and microbiomes · 2026Article
- Diverse Biological Functions and Domain-Specific Interactions of the Diguanylate Cyclase PA2072 inInternational journal of microbiology · 2026Article
- The diguanylate cyclase CrdA contributes to cell adhesion and gut colonization by switching motility and sessile state ofFrontiers in microbiology · 2026Article
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- A high-resolution genomic and phenotypic analysis of resistance evolution of anJournal of medical microbiology · 2025Article
- Linalool as a potential agent for inhibiting Escherichia coli biofilm formation and exopolysaccharide production.BMC veterinary research · 2025Article
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- Spatial propagation of temperate phages within and among biofilms.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Exploring Innovative Approaches to Isolate a One-Component c-di-GMP Transducer: A Pilot Study.Advances in experimental medicine and biology · 2025Article
- Structural basis for synthase activation and cellulose modification in the E. coli Type II Bcs secretion system.Nature communications · 2024Article
- A genetically encoded biosensor to monitor dynamic changes of c-di-GMP with high temporal resolution.Nature communications · 2024Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In many bacteria, the biofilm-promoting second messenger c-di-GMP is produced and degraded by multiple diguanylate cyclases (DGC) and phosphodiesterases (PDE), respectively. High target specificity of some of these enzymes has led to theoretical concepts of "local" c-di-GMP signaling. In Escherichia coli K-12, which has 12 DGCs and 13 PDEs, a single DGC, DgcC, is specifically required for the biosynthesis of the biofilm exopolysaccharide pEtN-cellulose without affecting the cellular c-di-GMP pool, but the mechanistic basis of this target specificity has remained obscure. DGC activity of membrane-associated DgcC, which is demonstrated in vitro in nanodiscs, is shown to be necessary and sufficient to specifically activate cellulose biosynthesis in vivo. DgcC and a particular PDE, PdeK (encoded right next to the cellulose operon), directly interact with cellulose synthase subunit BcsB and with each other, thus establishing physical proximity between cellulose synthase and a local source and sink of c-di-GMP. This arrangement provides a localized, yet open source of c-di-GMP right next to cellulose synthase subunit BcsA, which needs allosteric activation by c-di-GMP. Through mathematical modeling and simulation, we demonstrate that BcsA binding from the low cytosolic c-di-GMP pool in E. coli is negligible, whereas a single c-di-GMP molecule that is produced and released in direct proximity to cellulose synthase increases the probability of c-di-GMP binding to BcsA several hundred-fold. This local c-di-GMP signaling could provide a blueprint for target-specific second messenger signaling also in other bacteria where multiple second messenger producing and degrading enzymes exist.
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