ArticleNPJ biofilms and microbiomes2020
The zoonotic pathogen Leptospira interrogans mitigates environmental stress through cyclic-di-GMP-controlled biofilm production.
Article in NPJ biofilms and microbiomes, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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40 citing papers in PubMed, 58 citations in OpenAlex.
- Glutamine from plasma-like medium fuels nitrogen metabolism and influences proliferation in Leptospira interrogans.PLoS pathogens · 2026Article
- Identification ofMicroorganisms · 2026Article
- Multidrug-resistant and virulence traits in genetically distinct vesicular Leptospira inhabiting farmland and sewage in Southern India.Scientific reports · 2026Article
- Antimicrobial and antibiofilm activity of selected flavonoids against pathogenic Leptospira species.Archives of microbiology · 2026Article
- Environmental Detection of PathogenicPathogens (Basel, Switzerland) · 2026Article
- TheInfection and immunity · 2026Article
- Discovery of Novel c-di-GMP-Related Genes inPathogens (Basel, Switzerland) · 2026Article
- Divergent Fates of Hardjo Leptospires: Early Transcriptomic Response ofTransboundary and emerging diseases · 2026Article
- cGAS-STING dependent type I IFN reduces Leptospira interrogans renal colonization in mice.PLoS pathogens · 2026Article
- Protocols for Standard and Advanced Visualization and Quantification of Leptospira Biofilms.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Cultivation of Leptospira interrogans Within Rat Peritoneal Dialysis Membrane Chambers.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Exploring the impact of quercetin on the growth and biofilm formation of Leptospira interrogans.Folia microbiologica · 2025Article
- Evaluation of In Vitro Biofilm Formation of Leptospira Isolates From Human Samples at Four Different Time Frames.Cureus · 2025Article
- Article
- Host population dynamics influence Leptospira spp. transmission patterns among Rattus norvegicus in Boston, Massachusetts, US.PLoS neglected tropical diseases · 2025Article
- Review
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- Review
- Serovar-specific genomic features ofFrontiers in molecular biosciences · 2025Article
- Ketone body oxidation and susceptibility to ethyl acetoacetate in a novel hemolytic multidrug-resistant strain Leptospira interrogans KeTo originated from sewage water.Scientific reports · 2024Article
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Authors and funding
12 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The zoonotic bacterium Leptospira interrogans is the aetiological agent of leptospirosis, a re-emerging infectious disease that is a growing public health concern. Most human cases of leptospirosis result from environmental infection. Biofilm formation and its contribution to the persistence of virulent leptospires in the environment or in the host have scarcely been addressed. Here, we examined spatial and time-domain changes in biofilm production by L. interrogans. Our observations showed that biofilm formation in L. interrogans is a highly dynamic process and leads to a polarized architecture. We notably found that the biofilm matrix is composed of extracellular DNA, which enhances the biofilm's cohesiveness. By studying L. interrogans mutants with defective diguanylate cyclase and phosphodiesterase genes, we show that biofilm production is regulated by intracellular levels of bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di-GMP) and underpins the bacterium's ability to withstand a wide variety of simulated environmental stresses. Our present results show how the c-di-GMP pathway regulates biofilm formation by L. interrogans, provide insights into the environmental persistence of L. interrogans and, more generally, highlight leptospirosis as an environment-borne threat to human health.
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