ArticleBiomacromolecules2020
Synthesis and Characterization of Silk Ionomers for Layer-by-Layer Electrostatic Deposition on Individual Mammalian Cells.
Article in Biomacromolecules, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 39 citations in OpenAlex.
- Silk-Based Protein Corona Enhances mRNA-LNP Vaccine Efficacy and Prevents Tumor Relapse.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Methods for the fabrication of polyelectrolyte capsules for different biomedical applications.Discover nano · 2026Article
- Efficient and Rapid Microfluidics Production of Bio-Inspired Nanoparticles Derived fromPharmaceutics · 2025Article
- Selective Biofilm Inhibition through Mucin-Inspired Engineering of Silk Glycopolymers.Journal of the American Chemical Society · 2024Article
- Cross-Linking Methods of the Silk Protein Hydrogel in Oral and Craniomaxillofacial Tissue Regeneration.Tissue engineering and regenerative medicine · 2024Review
- Sustainable Silk-Based Particulate Systems for the Controlled Release of Pharmaceuticals and Bioactive Agents in Wound Healing and Skin Regeneration.International journal of molecular sciences · 2024Review
- Advancing cell surface modification in mammalian cells with synthetic molecules.Chemical science · 2023Review
- Application of silk fibroin coatings for biomaterial surface modification: a silk road for biomedicine.Journal of Zhejiang University. Science. B · 2023Review
- Multifunctional silk vinyl sulfone-based hydrogel scaffolds for dynamic material-cell interactions.Biomaterials · 2023Article
- Article
- Silk chemistry and biomedical material designs.Nature reviews. Chemistry · 2023Review
- Cytoprotection of Human Progenitor and Stem Cells through Encapsulation in Alginate Templated, Dual Crosslinked Silk and Silk-Gelatin Composite Hydrogel Microbeads.Advanced healthcare materials · 2022Article
- Review
- Sugar Functionalization of Silks with Pathway-Controlled Substitution and Properties.Advanced biology · 2021Article
- Synthesis of pH and Glucose Responsive Silk Fibroin Hydrogels.International journal of molecular sciences · 2021Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Nanocoating of individual mammalian cells with polymer layers has been of increasing interest in biotechnology and biomedical engineering applications. Electrostatic layer-by-layer (LbL) deposition of polyelectrolytes on negatively charged cell surfaces has been utilized for cell nanocoatings using synthetic or natural polymers with a net charge at physiological conditions. Here, our previous synthesis of silk-based ionomers through modification of silk fibroin (SF) with polyglutamate (PG) and polylysine (PL) was exploited for the nanocoating of mammalian cells. SF-PL constructs were cytotoxic to mammalian cells, thus an alternative approach for the synthesis of silk ionomers through carboxylation and amination of regenerated SF chains was utilized. Through the optimization of material properties and composition of incubation buffers, silk ionomers could be electrostatically assembled on the surface of murine fibroblasts and human mesenchymal stem cells (hMSCs) to form nanoscale multilayers without significantly impairing cell viability. The resulting silk-based protein nanoshells were transient and degraded over time, allowing for cell proliferation. The strategies presented here provide a basis for the cytocompatible nanoencapsulation of mammalian cells within silk-based artificial cell walls, with potential benefits for future studies on surface engineering of mammalian cells, as well as for utility in cell therapies, 3D printing, and preservation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.