ArticleMolecular systems biology2020
Controlling spatiotemporal pattern formation in a concentration gradient with a synthetic toggle switch.
Article in Molecular systems biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Travelling Waves in Gene Expression: A Mathematical Model of Cell-State Dynamics in Melanoma.Bulletin of mathematical biology · 2026Article
- A tool for modeling gene regulatory networks (GRN_modeler) and its applications to synthetic biology.Molecular systems biology · 2025Article
- A Quasi-Stationary Distribution Bound for Fault Analysis in Gene Regulatory Networks.bioRxiv : the preprint server for biology · 2025Article
- Contact area and tissue growth dynamics shape synthetic juxtacrine signaling patterns.Biophysical journal · 2025Article
- Automatic design of gene regulatory mechanisms for spatial pattern formation.NPJ systems biology and applications · 2024Article
- Design principles for synthetic control systems to engineer plants.Plant cell reports · 2023Review
- Article
- Toward predictive engineering of gene circuits.Trends in biotechnology · 2023Review
- Spatiotemporal Patterning Enabled by Gene Regulatory Networks.ACS omega · 2023Article
- Construction of the genetic switches in response to mannitol based on artificial MtlR box.Bioresources and bioprocessing · 2023Article
- The space between us: Modeling spatial heterogeneity in synthetic microbial consortia dynamics.Biophysical reports · 2022Article
- CRISPRi-based programmable logic inverter cascade for antibiotic-free selection and maintenance of multiple plasmids.Nucleic acids research · 2022Article
- Advances and challenges in programming pattern formation using living cells.Current opinion in chemical biology · 2022Review
- Synthetic spatial patterning in bacteria: advances based on novel diffusible signals.Microbial biotechnology · 2022Review
- Article
- A Framework for Investigating Rules of Life by Establishing Zones of Influence.Integrative and comparative biology · 2022Article
- Elucidating multi-input processing 3-node gene regulatory network topologies capable of generating striped gene expression patterns.PLoS computational biology · 2022Article
- Efficiency of the synthetic self-splicing RiboJ ribozyme is robust to cis- and trans-changes in genetic background.MicrobiologyOpen · 2021Article
- Time and space in segmentation.Interface focus · 2021Article
- Implications of diffusion and time-varying morphogen gradients for the dynamic positioning and precision of bistable gene expression boundaries.PLoS computational biology · 2021Article
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3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The formation of spatiotemporal patterns of gene expression is frequently guided by gradients of diffusible signaling molecules. The toggle switch subnetwork, composed of two cross-repressing transcription factors, is a common component of gene regulatory networks in charge of patterning, converting the continuous information provided by the gradient into discrete abutting stripes of gene expression. We present a synthetic biology framework to understand and characterize the spatiotemporal patterning properties of the toggle switch. To this end, we built a synthetic toggle switch controllable by diffusible molecules in Escherichia coli. We analyzed the patterning capabilities of the circuit by combining quantitative measurements with a mathematical reconstruction of the underlying dynamical system. The toggle switch can produce robust patterns with sharp boundaries, governed by bistability and hysteresis. We further demonstrate how the hysteresis, position, timing, and precision of the boundary can be controlled, highlighting the dynamical flexibility of the circuit.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.