Evidence map›Paper›PMID 32529777›Full record

ArticleThoracic cancer2020

Progesterone/Org inhibits lung adenocarcinoma cell growth via membrane progesterone receptor alpha.

Jian Xiao, Xi Chen, Xiaoxiao Lu, Mingxuan Xie, Bixiu He, Shuya He, Shaojin You, Qiong Chen

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Thoracic cancer, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
2.0field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 22 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Review
  9. Article
  10. Review
  11. Review
  12. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Jian XiaoNational Clinical Research Center for Geriatric Disorders, Xiangya Hospital of Central South University, Changsha, China.
Xi ChenDepartment of Respiratory and Critical Care Medicine, Xiangya Hospital of Central South University, Changsha, China.
Xiaoxiao LuNational Clinical Research Center for Geriatric Disorders, Xiangya Hospital of Central South University, Changsha, China.
Mingxuan XieNational Clinical Research Center for Geriatric Disorders, Xiangya Hospital of Central South University, Changsha, China.
Bixiu HeNational Clinical Research Center for Geriatric Disorders, Xiangya Hospital of Central South University, Changsha, China.
Shuya HeDepartment of Biochemistry and Molecular Biology, University of South China, Hengyang, China.
Shaojin YouLaboratory of Cancer Experimental Therapy, Histopathology Core, Atlanta Research & Educational Foundation (151F), Atlanta VA Medical Center , Emory University, Decatur, Georgia, USA.
Qiong ChenNational Clinical Research Center for Geriatric Disorders, Xiangya Hospital of Central South University, Changsha, China.ORCID 0000-0002-2534-8834
Central South University · CNEmory University · USUniversity of South China · CN

Funding

Clinical Research Fund of Xiangya Hospital 2016L02Hunan Provincial Clinical Medical Technology Innovation Guidance Project 2018SK52608National Natural Science Foundation of China 81572284National Natural Science Foundation of China 81602028National Natural Science Foundation of China 81770045
6 · The paper itself

Abstract

backgroundThe aim of this study was to determine whether progesterone could inhibit the growth of lung adenocarcinoma cells via membrane progesterone receptor alpha (mPRα) and elucidate its potential mechanism. The relationship between mPRα expression and the survival prognosis of lung adenocarcinoma patients was studied.

methodsA mPRα knockdown lung adenocarcinoma cell line was constructed and treated with P4 and Org (a derivative of P4 and specific agonist of mPRα). Cell proliferation was assessed using CCK-8 and plate colony formation assays. Protein expression was detected by western blotting. A nude mouse model of lung adenocarcinoma was established to assess the antitumor effect of P4/Org in vivo.

resultsWe initially determined that mPRα could promote the development of lung adenocarcinoma through the following lines of evidence. High expression of mPRα both at the mRNA and protein level was significantly associated with the poor prognosis of lung adenocarcinoma patients. The downregulation of mPRα inhibited the proliferation of lung adenocarcinoma cells. We further showed that mPRα mediates the ability of P4 to inhibit the growth of lung adenocarcinoma cells through the following lines of evidence: P4/Org inhibited the proliferation of lung adenocarcinoma cells; mPRα mediated the ability of P4/Org to inhibit lung adenocarcinoma cell proliferation; mPRα mediated the ability of P4/Org to inhibit the PKA (cAMP-dependent protein kinase)/CREB (cAMP responsive element binding protein) and PKA/β-catenin signaling pathways; and P4/Org inhibited the growth of a lung adenocarcinoma tumor model in vivo.

conclusionsIn summary, the results of our study show that progesterone can inhibit lung adenocarcinoma cell growth via mPRα.

Indexed as

Adenocarcinoma of LungAnimalsFemaleHumansMaleMiceProgesteroneReceptors, ProgesteroneTransfectionXenograft Model Antitumor AssaysProgesteroneReceptors, ProgesteroneAdenocarcinomalung cancermPRαprogesterone

Identifiers

PMID32529777
PMCPMC7396388
OpenAlexW3034521003

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.