ArticleMicrobial ecology2020
Shifts in Composition and Activity of Oral Biofilms After Fluoride Exposure.
Article in Microbial ecology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 30 citations in OpenAlex.
- Fluoridated milk is effective in prevention of enamel caries in adolescents: a randomised trial.Acta odontologica Scandinavica · 2025Trial
- Impact of sudanese chewing tobacco toombak on oral bacteria - a culture-based pilot study.Clinical oral investigations · 2026Article
- Fluoride varnish application and the temporal evolution of supragingival microbiota in children with differential caries risk.Journal of oral microbiology · 2026Article
- Delayed shifts in salivary microbiota composition following long-term use of 0.06% chlorhexidine toothpaste.Frontiers in oral health · 2026Article
- A novel dual-targeting strategy to suppress cariogenic bacteria and biofilms with engineered ARG/FBG bioactive composites.Regenerative biomaterials · 2026Article
- Severity-dependent alterations in oral microbiota and antibiotic resistance in children with dental fluorosis.Journal of oral microbiology · 2026Article
- Non-Thermal Atmospheric Plasma Enhances Biological Effects of Fluoride on Oral Biofilms.Journal of functional biomaterials · 2025Article
- Resin composite aggregated S-PRG particles are not superior to non-S-PRG under microcosm biofilm.Scientific reports · 2025Article
- Host sweet preference modulates the salivary microbiome and its divergent associations with plaque-associated and non-plaque-related oral diseases.Frontiers in microbiology · 2025Article
- Ion release dynamics of bioactive resin cement under variable pH conditions.Frontiers in oral health · 2025Article
- Unveiling the complexity of early childhood caries:Journal of oral microbiology · 2024Review
- Manipulating the diseased oral microbiome: the power of probiotics and prebiotics.Journal of oral microbiology · 2024Review
- Functional changes in the oral microbiome after use of fluoride and arginine containing dentifrices: a metagenomic and metatranscriptomic study.Microbiome · 2022Article
- The Oral Microbiota: Community Composition, Influencing Factors, Pathogenesis, and Interventions.Frontiers in microbiology · 2022Review
- Application of fluoride disturbs plaque microecology and promotes remineralization of enamel initial caries.Journal of oral microbiology · 2022Article
- Understanding dental caries as a non-communicable disease.British dental journal · 2021Article
- A Single Dose of Nitrate Increases Resilience Against Acidification Derived From Sugar Fermentation by the Oral Microbiome.Frontiers in cellular and infection microbiology · 2021Article
- Anti-microbial efficiency of gaseous ozone's combined use with fluoride and chlorhexidine on time-related oral biofilm: anMedical gas researchArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Oral diseases are biofilm-mediated diseases caused by imbalances in the ecology of resident microflora. Among them, dental caries (tooth decay) is considered the most common disease worldwide, and toothbrushing, which physically eliminates the oral biofilm, is the most widespread prevention strategy. Although it is well established that fluoride increases enamel resistance to acidic pH and promotes tooth remineralization, its effect on the biofilm bacterial communities' composition and metabolism is not fully understood. We have grown in vitro oral biofilms and used 16S rRNA Illumina sequencing to study the effect of fluoride on DNA- and RNA-based bacterial populations. In addition, a metatranscriptomic approach has also been performed, in which total RNA has been sequenced to study gene expression profiles in the presence/absence of 500 ppm sodium fluoride. Our data show a lower pH drop and a clear shift in total and metabolically active bacterial composition after fluoride exposure. Streptococcus oralis was the species most affected, with a 10-fold reduction in both DNA and RNA samples, whereas Rothia mucilaginosa underwent an 8-fold increase in the DNA and S. salivarius a 4- and 5-fold increase in the RNA and DNA samples, respectively. The metatranscriptomes indicated that fluoride exposure induced a dramatic shutdown of sugar metabolism, including significant under-expression of different sugar transporters, fucosidases, and a pyruvate oxidase, among others. The reduction in saccharolytic organisms and the inhibition of sugar fermentation pathways by fluoride may therefore be considered instrumental for the beneficial effect of fluoride-containing oral hygiene products.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.